S3917

Single-strand Binding Protein from Escherichia coli

>95% (SDS-PAGE), for molecular biology, Single strand binding protein to enhance PCR specificity

• Synonym(s): SSB
• MDL number: MFCD00213047
• UNSPSC Code: 12352202
• NACRES: NA.52

Properties

• grade: for molecular biology
• Quality Level: 200
• Assay: >95% (SDS-PAGE)
• form: liquid
• color: colorless
• foreign activity: DNase and RNase, none detected
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Single-strand Binding Protein is a homotetramer,[1] found in E. coli during the event of DNA metabolism.[2]

Application

Binds with high specificity to single-stranded DNA. Can be useful in enhancing the specificity of PCR and in enabling the sequencing of problematic DNA templates.

Single-strand Binding Protein from Escherichia coli has been used in in vitro RNA/Single-Strand Binding Protein (SSB) complex formation assays[3] and in protein purification to determine integrity and functionality of the integron attC sites.[4]

Biochem/physiol Actions

Single-strand Binding Protein (SSBP) exhibits high affinity towards single stranded DNA important for DNA metabolic processes.[1][5] Single-stranded DNA-binding protein hSSB1 is critical for genomic stability and assists the delivery of primers during the formation of lagging standard.[5] It also prevents the formation of inhibitory secondary structures in the exposed lagging-strand of DNA during replication.[5] SSBP mediates the recruitment of other proteins to their sites of function on DNA.[1]

Physical form

Solution in 20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1 mM EDTA, 0.1 mM DTT, 50% glycerol

Safety Information

• Pictograms: GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H318,H410
• Precautionary Statements: P273 - P280 - P305 + P351 + P338 - P391 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2