The protein content and enzymatic activity of this product vary from lot to lot. The protein content will range from 20% - 40%, and the activity will range from 10 units - 50 units per milligram of protein. As an example, a batch that reports a protein content of 20% and an activity of 10 units per milligram of protein will have a vial content of 12.5 mg for the 25 unit package size. The protein content and activity are reported in the product Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/p7633#product-documentation
P7633
Phospholipase C from Clostridium perfringens (C. welchii)
Type I, lyophilized powder, 10-50 units/mg protein
Synonym(s):
Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase
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25 UNITS
€60.80
125 UNITS
€190.00
500 UNITS
€567.00
€60.80
Please contact Customer Service for Availability
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25 UNITS
€60.80
125 UNITS
€190.00
500 UNITS
€567.00
About This Item
€60.80
Please contact Customer Service for Availability
Recommended Products
biological source
bacterial (Clostridium perfringens)
Quality Level
type
Type I
form
lyophilized powder
specific activity
10-50 units/mg protein
composition
protein, 20-40%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
application(s)
diagnostic assay manufacturing
greener alternative category
, Enabling
storage temp.
−20°C
Gene Information
Clostridium perfringens str. 13 ... plc(988262)
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General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Application
Phospholipase C (PLC) has been used to study adrenoceptor-mediated transmembrane signaling. It is also used to degrade inositol-containing phospholipids. Furthermore, it has been used to study 9E3 gene activation[1].
Biochem/physiol Actions
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol. This enzyme also hydrolyzes the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids. Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid.
Unit Definition
One unit will liberate 1.0 μmole of water soluble organic phosphorus from egg yolk L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.
inhibitor
Product No.
Description
Pricing
substrate
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Certificates of Analysis (COA)
Lot/Batch Number
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Customers Also Viewed
Mojtaba Alimolaei et al.
Probiotics and antimicrobial proteins, 10(2), 251-257 (2017-04-13)
The alpha-toxin is one of the virulence factors of Clostridium perfringens for gas gangrene in humans and animals or necrotic enteritis in poultry. The C-terminal domain of this toxin ( cpa 247-370 ) was synthesized and cloned into pT1NX vector
R Spangler et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(18), 7017-7021 (1989-09-01)
Induction of the transformation-related gene 9E3 by the v-src and v-fps gene products (v-Src and v-Fps) is blocked in chicken embryo fibroblasts depleted of protein kinase C (PKC). PKC agonists induce 9E3 gene expression. Protein kinase inhibitors block v-Src- and
Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein.
T Takahashi et al.
Biochimica et biophysica acta, 351(1), 155-171 (1974-05-10)
N Sieger et al.
Arthritis and rheumatism, 65(3), 770-779 (2012-12-13)
CD22 is a surface molecule exclusively expressed on B cells that regulates adhesion and B cell receptor (BCR) signaling as an inhibitory coreceptor of the BCR. Central downstream signaling molecules that are activated upon BCR engagement include spleen tyrosine kinase
Zhou Zhou et al.
Circulation, 127(4), 476-485 (2012-12-26)
Platelet hyperactivity induced by inflammation is a known risk factor for atherosclerosis and thrombosis, but its underlying mechanisms remain poorly understood. The signal transducer and activator of transcription 3 (STAT3) was activated in collagen-stimulated platelets. Activated STAT3 served as a
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what is the meaning of 10-50 units/mg protein.
1 answer-
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P7633とP4039の精製方法の概要（方法名のみで差し支えございません）を開示して頂くことは可能でしょうか？
1 answer-
Unfortunately, the purification method for these products are considered proprietary. However, the following reference may be of interest:
Purification of Clostridium perfringens phospholipase C (alpha-toxin) by affinity chromatography on agarose-linked egg-yolk lipoprotein. T Takahashi et al. Biochimica et biophysica acta, 351(1), 155-171 (1974-05-10)
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What are the differences in the purification methods between Products P7633 and P4039 Phospholipase C from Clostridium perfringens (C. welchii)?
1 answer-
The extraction and purification methods for our Phospholipase C products are proprietary, however higher unit per milligram values indicate a more purified product. Product P7633 contains a specific activity of 10-50 units/mg protein and the protein content is between 20.0 - 40.0 %. Product P4039 will contain a specific activity of ≥150 units/mg protein. The protein content for this product is between 5.0 - 30.0 %. The impact of the methods on the isoform profile of these products is not evaluated. The specific activity of each lot activity will be reported in the Certificate of Analysis. Please view the sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P7633-500UN0000218762.pdf
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P4039-125UN0000185061.pdfHelpful?
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Are Products P7633 and P4039, Phospholipase C from Clostridium perfringens (C. welchii), prepared from the same strain?
1 answer-
Products P7633 and P4039, Phospholipase C, from Clostridium perfringens (C. welchii) are prepared from the same strain.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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