The identity of the substrate is proprietary and cannot be disclosed. The kit can work for both GDP1 and GDP2.
MAK208
GPDH Activity Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
Glycerol 3-Phosphate Dehydrogenase Activity Assay Kit
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.84
Recommended Products
detection method
colorimetric
storage temp.
−20°C
Gene Information
human ... GPD1(2819) , GPD2(2820)
mouse ... GPD1(14555) , GPD2(14571)
rat ... GPD1(60666) , GPD2(25062)
General description
Glycerol 3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) catalyzes the reversible conversion of dihydroxyacetone phosphate and NADH to glycerol-3-phosphate and NAD+.[1][2] It forms a glycerol phosphate shuttle and facilitates the transfer of reduced equivalents across the mitochondria to the cytosol.[3] GPDH is important for both carbohydrate and lipid metabolism.[2] Mutations in GPDH gene result in transient infantile hypertriglyceridemia.[1]
Application
GPDH (glyceraldhyde-3-phoshate dehydrogenase) activity assay kit has been used to measure the serum and hepatic concentrations of GPDH in rabbit.[4]
Suitability
Suitable for the detection of Glycerol-3-Phosphate Dehydrogenase Activity in tissue and cell culture samples.
Principle
The Glycerol 3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit provides a simple and sensitive procedure for measuring GPDH activity in a variety of tissues and cells. GPDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of GPDH is the amount of enzyme required to generate 1.0 μmole of NADH per minute at pH 8 at 37 °C.
Kit Components Only
Product No.
Description
- GPDH Assay Buffer
- GPDH Substrate
- GPDH Probe
- NADH Standard
- GPDH Positive Control
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
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A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.
Joshi M, et al.
European Journal of Human Genetics, 22(10), 1229-1229 (2014)
B. Elmahdi, MA Al-Omair, AA El-Bessoumy and SM El-Bahr.
Thomson I S I
International Journal of Pharmaceutics, 12(4), 429-434 (2016)
Regulation of adenine nucleotide translocase and glycerol 3-phosphate dehydrogenase expression by thyroid hormones in different rat tissues.
DUMMLER K, et al.
The Biochemical Journal, 317(3), 913-918 (1996)
Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 ? resolution.
Alarcon D A, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 68(11), 1279-1283 (2012)
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What is the substrate used for detection in this kit? The description indicates that it can be used for both GPD1 and GPD2 (mitochondrial GPDH) activity detection.
1 answer-
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Which substrate is used for the detection in the activity assay kit for mGPDH (GPD2)
1 answer-
The substrate used for detection in the MAK208 kit for measuring Glycerol 3-phosphate dehydrogenase (GPDH) activity is dihydroxyacetone. This kit specifically measures cytosolic GPDH, identified by the EC number 1.1.1.8, and it quantifies the production of NADH. Cytosolic GPDH catalyzes the conversion of sn-glycerol 3-phosphate and NAD into NADH, glycerone phosphate, and H+. On the other hand, mitochondrial GPDH, identified by the EC number 1.1.5.3, catalyzes a different reaction involving sn-glycerol 3-phosphate and quinone into glycerone phosphate and quinol.
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