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G1910

Sigma-Aldrich

Gelzan CM

Gelrite®

Synonym(s):

Gellan Gum, Agar substitute gelling agent

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250 G
€233.00
1 KG
€753.00
5 KG
€3,400.00

€233.00


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250 G
€233.00
1 KG
€753.00
5 KG
€3,400.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
10171502
NACRES:
NA.72

€233.00


Please contact Customer Service for Availability

Request a Bulk Order

Quality Level

application(s)

agriculture

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General description

Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological assay and tissue culture media. Gellan gum is used in a wide variety of immobilization matrices. It forms a hard gel at low concentrations in the presence of an electrolyte. Gellan is a linear tetrasaccharide composed of D-glucose, L-rhamnose, and D-glucuronic acid in the ratio of 2:1:1, linked by β (1 → 4) glycosidic bond.
Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological assay and tissue culture media. Gellan gum is used in a wide variety of immobilization matrices. It forms a hard gel at low concentrations in the presence of an electrolyte. Gellan is a linear tetrasaccharide composed of D-glucose, L-rhamnose, and D-glucuronic acid in the ratio of 2:1:1, linked by β (1 → 4) glycosidic bond.

Application

Gelrite, gellan gum, is recommended as a gelling agent for use in place of agar in microbiological media. Gellan gum is used in a wide variety of immobilization matricies.
Gelzan CM has been used:
  • in the synthesis of gellan-pullulan nanogel via chemical crosslinking
  • in the synthesis of gellan-pullulan hydrogel
  • with KNOP medium to maintain and prepare Anthoceros agrestis thallus tissue sample for imaging

Gelzan CM has been used:
  • in the synthesis of gellan-pullulan nanogel via chemical crosslinking[1]
  • in the synthesis of gellan-pullulan hydrogel[2]
  • with KNOP medium to maintain and prepare Anthoceros agrestis thallus tissue sample for imaging[3]

Features and Benefits

  • Transparent and colorless agar alternative
  • Mechanically robust
  • Disperses in water with ease
  • Low viscosity
  • The resulting gel has a high melting temperature

Legal Information

Gelrite is a registered trademark of CP Kelco U.S., Inc.
Gelzan is a trademark of CP Kelco U.S., Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

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Caroline Teyssier et al.
Physiologia plantarum, 141(2), 152-165 (2010-10-26)
An integrated physiological and proteomic approach was used to investigate the effects of high gellan gum concentration in the medium during maturation of somatic embryos (SE) of hybrid larch, by comparing embryos incubated in media with a high gellan gum
Valeria Imperato et al.
Genes, 10(6) (2019-06-20)
Hydrocarbon-degrading bacteria are important resources for use in phytoremediation applications. Yet, for many hydrocarbonoclastic strains the genetic information regarding pollutant degradation and detoxification has not been thoroughly revealed. In this study, hydrocarbon-degrading bacteria were isolated from a long-term oil-polluted soil
Gustavo de Medeiros et al.
Nature communications, 6, 8881-8881 (2015-11-26)
Selective-plane illumination microscopy has proven to be a powerful imaging technique due to its unsurpassed acquisition speed and gentle optical sectioning. However, even in the case of multiview imaging techniques that illuminate and image the sample from multiple directions, light
Marco A Lopez-Heredia et al.
Journal of tissue engineering and regenerative medicine, 12(8), 1825-1834 (2018-04-28)
Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO3 is a widely used bone regeneration material, and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone-forming cell adhesion and proliferation and bone regeneration.
Timothy E L Douglas et al.
Journal of biomedical materials research. Part A, 106(3), 822-828 (2017-10-24)
Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles

Protocols

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Questions

1–10 of 11 Questions  
  1. Does Gellan Gum/Gelrite have Iron, Manganese and Zinc in trace amount? It would be great to have the specific concentration detected. Thank you.

    1 answer
    1. Unfortunately, the levels of Iron, Manganese and Zinc are not analyzed for this product.

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  2. How many grams per liter of Product G1910, Gelzan™ CM are recommended for plant tissue culture?

    1 answer
    1. Gelzan™ CM is typically used at a concentration of 2-4 grams per liter.

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  3. Buonasera, sarebbe possibile sapere il grado di purezza del Gelzan? visto che nel Gelzan ci sarebbero sali o altri componenti, quanto è la concentrazione del Gellan Gum nel Gelzan? Rimango in attesa di un vostro riscontro.

    1 answer
    1. The purity of this gelling agent is not determined. This material is a complex polysaccharide comprised of glucose, rhamnose, and glucuronic acid in the approximate molar ratio of 2:1:1. In addition to the base components, historical ICP analysis shows trace amounts of Calcium, Magnesium, Potassium, Phosphate, and Sodium. The maximum moisture content for this product is 16%. Please see the link below to review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G1910-BULK0000254218.pdf

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  4. How do I use Product G1910, Gelzan™ CM?

    1 answer
    1. The G1910 is added to well-stirred water, and the dispersion heated to 85-90°C to ensure full hydration. To form a gel, the necessary amount of electrolyte, such as potassium citrate or calcium chloride, is added to the solution at 85°C and the mixture is poured or pumped into containers and allowed to cool undisturbed. The product is biodegradable, so a biocide is recommended. The typical use level is 0.2 to 2.0%.

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  5. Is Product G1910, Gelzan™ CM, different that Gelrite?

    1 answer
    1. The G1910 is chemically the same as Gelrite, which is no longer produced. Both items are trademarked to CP Kelco U.S., Inc. They are both Gellan Gum, but the manufacturer has different specifications for these items.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. What is Product G1910, Gelzan CM?

    1 answer
    1. It is a low-acyl gellan gum used as a gelling agent in both microbiological assay media and tissue culture.

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  8. What is the difference between Product G1910, Gelzan CM and Gelrite?

    1 answer
    1. The products are chemically the same. However, Gelzan CM is produced through a new and more efficient process. There are some slight differences in the specifications of the two products.

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  9. Does Product G1910, Gelzan CM, have an expiration date?

    1 answer
    1. The lots of this product are not assigned expiration dates. The guarantee of quality is one year from the time of delivery for unopened bottles stored at room temperature.

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  10. What is the gel strength of Product G1910, Gelzan™ CM?

    1 answer
    1. The gel strength as determined by the vendor is between 400-700 g/cm2.

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1–10 of 11 Questions  

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