We test the coated culture surface by culturing cells. We compare the adherence of the cells to wells that have been coated with media containing BSA (negative) and media containing 10% FBS (positive). The cell growth should be not less than 50% of the positive control and greater than 50% of the negative control.
F2006
Human Fibronectin
from human plasma, powder, suitable for cell culture
Synonym(s):
Fibronectin
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1 MG
€425.00
2 MG
€788.00
5 MG
€1,410.00
5 X 5 MG
€3,640.00
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1 MG
€425.00
2 MG
€788.00
5 MG
€1,410.00
5 X 5 MG
€3,640.00
About This Item
Recommended Products
Product Name
Fibronectin human plasma, lyophilized powder, BioReagent, suitable for cell culture
biological source
human plasma
Quality Level
sterility
sterile
product line
BioReagent
Assay
≥85% (SDS-PAGE)
form
lyophilized powder
mol wt
450 kDa
packaging
pkg of 5 x 5 mg
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
concentration
>1 mg/mL protein
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
impurities
HBSAG, none detected
HCV, none detected
HIV-1/HIV-2, none detected
solubility
H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
NCBI accession no.
UniProt accession no.
Binding Specificity
Peptide Source: Collagen
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... FN1(2335)
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General description
Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous in nature and form functional unit arranged linearly on two subunit arms.[1] It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of fibronectin protein due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.[2]
Application
Fibronectin from human plasma is used for the following applications:
- Used in cell seeding and neuronal differentiation (as one of the components in expansion medium for the re-suspension of CBSCs (Cord blood stem cells) and is also used in differentiation medium)[3]
- Used in cell culture (the culture chamber was coated with fibronectin for the expression of HEK293 cells)[4]
- In-vitro fibronectin (FN) pulldown assays[5]
- Protein specificity[6]
- Used in cell migration study[7]
Biochem/physiol Actions
Fibronectin (FN) plays an essential role in multiple biological functions such as, angiogenesis, cell migration cell-to-cell adhesion, cell-to-basement-membrane attachment, clot stabilization, embryogenesis, differentiation, wound healing, as well as binding of pathogen to mammalian cells and extracellular matrix. [1] Studies in Chinese Han population show that mutations in this gene might be linked with susceptibility to knee osteoarthritis (OA).[2] Splice variant extra domain-B (EDB) of FN is a hallmark of tumor angiogenesis, and with increased matrix stiffness there is an increase in the production of this isoform by endothelial cells.[8] In colorectal cancer (CRC), EDA is linked with metastasis, vasculogenesis and tumorigenesis and promotes epithelial-mesenchymal transition.[9]
Caution
The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.
Preparation Note
This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B, hepatitis C and syphilis. In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Protocols
Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.
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How do I test the wells/flasks that have been coated with fibronectin?
1 answer-
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How do I determine how much fibronectin is in solution?
1 answer-
As the product may contain undissolved material, the amount of fibronectin in solution can be determined by UV absorbance. The extinction coefficient for a 1% solution (10 mg/ml) is 13.5 at 280 nm.
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What is the procedure for coating culture surfaces with fibronectin?
1 answer-
To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume. 2. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but this is not necessary.
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How do I solubilized Fibronectin?
1 answer-
Dissolve at 1 mg/mL in water. Allow to dissolve for at least 30 minutes at 37 °C. A small amount of undissolved material may remain. This will not affect product performance.
Helpful?
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How do I choose which fibronectin product to use to coat culture surfaces for cell growth?
1 answer-
The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
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How long can I store fibronectin coated plates, etc?
1 answer-
Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
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What is the difference between Fibronection Products F2006 and F1056?
1 answer-
The difference between Product Nos. F2006 and F1056 is in the purity specifications and endotoxin testing. Product No. F2006 has a purity specification of ≥90% and is not tested for endotoxin contamination. Product No. F1056 has a purity specification of ≥95% and is tested for endotoxin contamination with a specification of ≤100 EU/mg.
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How long can I store the fibronectin solution?
1 answer-
The product is good for at least a year at 2-8 °C if kept sterile.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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