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EXTRA2

Sigma-Aldrich

Mouse ExtrAvidin® Peroxidase Staining Kit antibody produced in goat

Synonym(s):

ExtrAvidin® Peroxidase Staining Kits

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.32

antibody product type

secondary antibodies

usage

sufficient for 2,000 tests ELISA, Immunoblotting
 sufficient for 200 tests Immunohistology

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

These kits comprise universal reagents for use with primary antibodies in immunohistology, ELISA, and immunoblotting.
ExtrAvidin® is a unique form of avidin, available only from Sigma, that combines the high specificity and affinity of avidin for biotin with low non-specific binding at physiological pH. ExtrAvidin® peroxidase exhibits high sensitivity with low background.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Features and Benefits

  • Use in immunohistology, ELISA, and immunoblotting assays.
  • Affinity Isolated Antibodies have been adsorbed with human IgG and IgM to minimize cross-reactivity.
  • Biotinylated antibodies contain a spacer which improves accessibility for the ExtrAvidin® conjugates.

Components

• 3 mL ExtrAvidin®-Peroxidase
• 3 mL Biotinylated Secondary Antibodies
• Complete Instructions and Assay Protocols

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Matheus L Medeiros et al.
Journal of inflammation research, 14, 6477-6489 (2021-12-10)
Methylglyoxal (MGO) is a highly reactive dicarbonyl species implicated in diabetic-associated diseases. Acute lung injury (ALI) symptoms and prognosis are worsened by diabetes and obesity. Here, we hypothesized that elevated MGO levels aggravate ALI, which can be prevented by metformin.
Akila L Oliveira et al.
Frontiers in physiology, 13, 860342-860342 (2022-04-15)
Methylglyoxal (MGO) is a highly reactive dicarbonyl compound implicated in diabetes-associated diseases. In vascular tissues, MGO induces the formation of advanced glycation end products (AGEs) that bounds its receptor RAGE, initiating the downstream tissue injury. Outside the cardiovascular system, MGO
Vladimirs Pilipenko et al.
Neuropharmacology, 144, 319-326 (2018-11-09)
Early manifestations of Alzheimer's disease (AD) include neuroinflammation, disrupted neurotransmission and cognitive deficits. Impairment of the GABAergic system is essentially involved in the pathogenesis of AD. Traditionally, agonists of GABAA receptors at doses above 1 mg/kg are known to possess memory

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