D9281

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

• UNSPSC Code: 41141827
• NACRES: NA.25

Properties

• Quality Level: 100; 200
• form: liquid
• usage: 100 uses (0.1 mg size); 500 uses (0.5 mg size)
• suitability: suitable for electrophoresis (DNA)
• storage temp.: −20°C

Description

General description

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.[1]

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Components

Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 13 fragments, 125–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable