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C0954

Sigma-Aldrich

Chondroitinase C from Flavobacterium heparinum

lyophilized powder, ≥200 units/mg solid

Synonym(s):

Chondroitin C lyase

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About This Item

CAS Number:
60184-91-0
MDL number:
MFCD00212904
UNSPSC Code:
12352204
NACRES:
NA.54

conjugate

(Glucosaminoglycan)

Quality Level

200

form

lyophilized powder

specific activity

≥200 units/mg solid

storage temp.

−20°C

Related Categories

General description

Enzyme Commission (EC) Number 4.2.2.x

Biochem/physiol Actions

Chondroitinase C acts upon chondroitin sulfate C, releasing unsaturated 6-sulfated disaccharides. The enzyme also acts on hyaluronic acid, producing unsaturated nonsulfated disaccharides. The enzyme is strongly inhibited by NaCl, Fe2+, Co2+ and phosphate ions.

Unit Definition

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 8.0 at 25 °C using chondroitin sulfate C as substrate.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Y M Michelacci et al.
The Journal of biological chemistry, 251(4), 1154-1158 (1976-02-25)
A chondroitinase that acts upon chondroitin sulfate C and hyaluronic acid was isolated from Flavobacterium heparinum. This enzyme was seperated from constitutional chondroitinase AC and an induced chondroitinase B also present in extracts of F. heparinum previously grown in the
N Ototani et al.
Carbohydrate research, 70(2), 295-306 (1979-05-01)
Chondroitinase B and chondroitinase C were separated from an extract of Flavobacterium heparinum induced with chondroitin 6-sulfate by using column chromatography on hydroxylapatite. Chondroitinase C was eluted together with the activities of hyaluronidase, delta4,5glycosiduronase, and sulfatase. The latter two activities

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