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B0174

Sigma-Aldrich

AccuTaq™ LA 10× Buffer

10X Buffer for long and accurate PCR

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About This Item

UNSPSC Code:
41106306
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grade

Molecular Biology
for molecular biology

Quality Level

200

form

liquid

packaging

vial of 0.5 mL

concentration

10 ×

technique(s)

PCR: suitable

storage temp.

−20°C

Related Categories

General description

AccuTaq™ LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase.

Application

AccuTaq™ LA 10× Buffer has been used:

  • in PCR reaction mixture for the amplification of human BRCA1 gene.[1]
  • as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum[2]
  • as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV)[3]

Legal Information

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
0000295519
0000295519
SLCP4419
SLCF8408
SLCB2592

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Hameer Ruparel et al.
Genome research, 14(2), 296-300 (2004-02-06)
Deletion or insertion mutations lead to a frameshift that causes misalignment between wild-type and mutated allele sequences, making it difficult to identify such mutations unambiguously by using electrophoresis-based DNA sequencing. We have previously established the feasibility of an accurate DNA
Vandana Jaiswal et al.
PloS one, 10(6), e0129400-e0129400 (2015-06-16)
TaGW2 is an orthologue of rice gene OsGW2, which encodes E3 RING ubiquitin ligase and controls the grain size in rice. In wheat, three copies of TaGW2 have been identified and mapped on wheat homoeologous group 6 viz. TaGW2-6A, TaGW2-6B
Arunava Sarkar et al.
Biologicals : journal of the International Association of Biological Standardization, 48, 10-23 (2017-06-25)
In this study, the efficiency of binary ethyleneimine (BEI) in combination with formaldehyde (FA) and glutaraldehyde (GTA) in inactivating the Indian FMDV vaccine strains is compared. The acceptable safety of virus inactivation was faster and the inactivation rates were increased
Jeong-Yun Choi et al.
The Journal of biological chemistry, 286(36), 31180-31193 (2011-07-26)
The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts. Both mRNAs and proteins of all four DNA
Baozhong Meng et al.
The Journal of general virology, 86(Pt 5), 1555-1560 (2005-04-16)
Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have
View All Related Papers

Protocols

Long & Accurate PCR with RedAccuTaq®

REDAccuTaq LA protocol offers high-fidelity amplification of long PCR fragments with direct gel loading capability.

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