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A9415
Actinomycin D
from Streptomyces sp., suitable for cell culture, ≥95%
Synonym(s):
Cosmegen, Meractinomycin, Actinomycin C1, Actinomycin IV, Dactinomycin
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2 MG
€72.10
5 MG
€111.00
10 MG
€213.00
25 MG
€465.00
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2 MG
€72.10
5 MG
€111.00
10 MG
€213.00
25 MG
€465.00
About This Item
Empirical Formula (Hill Notation):
C62H86N12O16
CAS Number:
Molecular Weight:
1255.42
Beilstein:
605235
EC Number:
MDL number:
UNSPSC Code:
51282001
PubChem Substance ID:
NACRES:
NA.76
Recommended Products
biological source
Streptomyces sp.
Quality Level
Assay
≥95%
form
powder
technique(s)
cell culture | mammalian: suitable
color
red, powder
mp
251-253
solubility
ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)
antibiotic activity spectrum
neoplastics
Mode of action
DNA synthesis | interferes
storage temp.
2-8°C
SMILES string
CC(C)[C@H]1NC(=O)[C@@H](NC(=O)c2ccc(C)c3OC4=C(C)C(=O)C(N)=C(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]6CCCN6C(=O)[C@H](NC5=O)C(C)C)C4=Nc23)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]7CCCN7C1=O
InChI
1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1
InChI key
RJURFGZVJUQBHK-IIXSONLDSA-N
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General description
Chemical structure: peptide
Application
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells.. Recommended for us in cell culture applications at 1μg/mL.
Biochem/physiol Actions
Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.
Caution
This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.
Preparation Note
Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.
Other Notes
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Oral - Carc. 1B - Repr. 1B
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Protocols
WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.
XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
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What is the Department of Transportation shipping information for this product?
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What is the working concentration for Actinomycin D (Product No. A9415)?
1 answer-
For cell culture applications, actinomycin D is used as a selection agent and is used in banding techniques to differentiate between different regions of chromosomes. Suggested working concentrations is 1 μg/mL.
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How do you recommend dissolving Product No. A9415 (Actinomycin D)?
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Stock solutions of this product may be prepared at 10 mg/mL in either ethanol (EtOH) or dimethyl sulfoxide (DMSO).
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How long are stock solutions of Actinomycin D (Product No. A9415) stable?
1 answer-
Stock solutions of this product in DMSO or ethanol should be stored in foil-wrapped vials at -20 °C. In DMSO, solution can be stored for up to 1 month at -20 °C. Do not store in glass vials.
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