A1733

AMPK (α2/β1/γ1), active, His tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): AMK2, AMPKG, HAMPKb, MGC17785, MGC8666, PRKAA2, PRKAB1, PRKAG1
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• product line: PRECISIO^® Kinase
• Assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 230-311 nmol/min·mg
• mol wt: A2 subunit ~69 kDa; B1 subunit ~38 kDa; G1 subunit ~40 kDa
• UniProt accession no.: P54619; P54646; Q9Y478
• relevant disease(s): diabetes
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PRKAA2(5563) , PRKAB1(5564) , PRKAG1(5571)

Description

Biochem/physiol Actions

AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes. AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization.

Physical form

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H360D
• Precautionary Statements: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves