61048
Abberior® STAR 488, NHS ester
for STED application
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
form
solid
concentration
≥50.0% (degree of coupling)
solubility
DMF: 1 mg/mL, clear
fluorescence
λex 510 nm; λem 514-534 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Abberior STAR 488 is the latest development for STED microscopy. It is a very bright green fluorescent dye that can be very effectively excited at the prominent 488 nm laser line. Abberior STAR 488 can substitute dyes like Oregon Green 488, Atto™ 488 or Alexa Fluor 488.
STED: The dye can most effectively be depleted in STED microscopy at 585-605 nm.
Abberior STAR 488 is the dye of choice for excitation at 488 nm. Even more, the dye is particularly signed and tested for 2-color STED microscopy in combination with our STAR 440SX rusing a 590nm laser. The dye has been very successfully tested in the Leica 2-color TCS STED CW microscope.
Key Features
Absorption Maximum, λmax: 503 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 65′000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.28 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.14 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 524nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 585 - 605 nm
Fluorescence Quantum Yield, η: 0.89 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.9 ns (MeOH)
STED: The dye can most effectively be depleted in STED microscopy at 585-605 nm.
Abberior STAR 488 is the dye of choice for excitation at 488 nm. Even more, the dye is particularly signed and tested for 2-color STED microscopy in combination with our STAR 440SX rusing a 590nm laser. The dye has been very successfully tested in the Leica 2-color TCS STED CW microscope.
Key Features
- STED dye of choice at 488 nm excitation
- Ideal depletion behavior in STED microscopy ~590 nm
- 2-color labeling partner with STAR 440SX for 2-color STED microscopy
Absorption Maximum, λmax: 503 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 65′000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.28 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.14 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 524nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 585 - 605 nm
Fluorescence Quantum Yield, η: 0.89 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.9 ns (MeOH)
Application
Anti-Rabbit IgG-Abberior® STAR 488 antibody has been used as a secondary antibody:
- for fluorescent immunohistochemistry in prostate cancer cell lines, PPC-1 and TSU-Pr1
- for immunofluorescence in HeLa and monkey kidney COS-7 cells
- for STED (stimulated emission depletion) microscopy in OLN-t40-α-syn cells (oligodendroglial cells)
Suitability
Designed and tested for fluorescent super-resolution microscopy
Other Notes
Legal Information
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Methods (San Diego, Calif.), 66(2), 124-130 (2013-07-03)
Stimulation emission depletion (STED) microscopy breaks the spatial resolution limit of conventional light microscopy while retaining its major advantages, such as working under physiological conditions. These properties make STED microscopy a perfect tool for investigating dynamic sub-cellular processes in living
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Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
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