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3011

Sigma-Aldrich

Transcreener® ADP2 TR-FRET Red Assay

Synonym(s):

Transcreener assay

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1 EACH
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About This Item

UNSPSC Code:
41122100
NACRES:
NA.84

€1,000.00

Please contact Customer Service for Availability

shipped in

dry ice

storage temp.

−20°C

General description

The Transcreener® ADP2 TR-FRET Red Assay is a competitive immunoassay for ADP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for ADP, the assay can be used with any enzyme that converts ATP to ADP, regardless of what other substrates are used. Examples of enzymes include protein, lipid, and carbohydrate kinases, ATPases, DNA helicases, carboxylases, and glutamine synthetase.
The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® ADP2 TR-FRET Red Assay provides the following benefits:
  • Accommodates ATP concentrations ranging from 0.1 μM to 1,000 μM. • Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10% or less).
  • Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
  • Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
  • Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

View full Transcreener® product list

Quantity

3011-1K = 1,000 assay, 384-well
3011-10K = 10,000 assay, 384-well

Physical form

Kit with buffered aqueous solutions

Legal Information

Transcreener is a registered trademark of BellBrook Labs

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Certificates of Analysis (COA)

Lot/Batch Number
13642

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Wendy R Sorenson et al.
Journal of AOAC International, 90(3), 670-678 (2007-06-22)
An interlaboratory study was conducted to evaluate a method for the determination of campesterol, stigmasterol, and beta-sitosterol in saw palmetto raw materials and dietary supplements at levels >1.00 mg/100 g based on a 2-3 g sample. Test samples were saponified
D N Angelov et al.
Developmental neuroscience, 20(1), 42-51 (1998-05-26)
Embryonic stem (ES) cells of the permanent line BLC6 derived from a 129/Sv Gat mouse blastocyst were differentiated as spheroid aggregates (embryoid bodies, EBs) in the presence of retionic acid. After 2 days in suspension, EBs were plated on gelatine-coated

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