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Key Documents

09969

Sigma-Aldrich

SQ-NHS

suitable for fluorescence, >50% (coupling to amines)

Synonym(s):

Squarain-carboxylate N-succinimidyl ester

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About This Item

Empirical Formula (Hill Notation):
C34H32ClN3O6
CAS Number:
Molecular Weight:
614.09
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

Assay

>50% (coupling to amines)

solubility

DMF: soluble

fluorescence

λex 600 nm; λem 645 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

2-8°C

InChI

1S/C34H32ClN3O6/c1-7-37-25-10-8-18(32(43)44-38-28(39)12-13-29(38)40)14-22(25)34(4,5)27(37)17-21-30(41)20(31(21)42)16-26-33(2,3)23-15-19(35)9-11-24(23)36(26)6/h8-11,14-17H,7,12-13H2,1-6H3

InChI key

RRLJXAKQFSXTLC-UHFFFAOYSA-N

Application

SQ-NHS contains a reactive N-hydroxysuccinimide group, which allows for coupling with amino groups. When conjugated with proteins, SQ-NHS demonstrates a high quantum yield with an absorption maximum at 640 nm and a nanosecond lifetime. Additionally, in the physiological pH range (6-9), the absorbance, emission, and lifetime of SQ-NHS are pH-independent. It is water insoluble .

related product

Product No.
Description
Pricing

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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