11758241001

β-Gal Reporter Gene Assay, chemiluminescent

sufficient for 500 assays (microplate), sufficient for 250 assays (tubes)

• Synonym(s): galactosidase
• UNSPSC Code: 23201100

Properties

• usage: sufficient for 250 assays (tubes); sufficient for 500 assays (microplate)
• manufacturer/tradename: Roche
• storage temp.: 2-8°C

Description

General description

The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid.

Application

The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene.

Features and Benefits

• Sensitive: Chemiluminescence technology allows detection of approximately 20 fg β-galactosidase in cell extracts
• High dynamic measuring range: Linear range over four to five orders of magnitude
• Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
• Safe: No radioactive isotopes are used
• Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
• Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control

Packaging

1 kit containing 7 components.

Preparation Note

Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

• β-Gal Substrate, Galacton Plus 100x concentrated
• Assay Buffer
• Enhancer
• Initiation Solution
• Protease Inhibitors
• Lysis Buffer 5x concentrated
• Positive Control, β-galactosidase (E. coli), lyophilizate

Safety Information

• Pictograms: GHS02,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H290,H314,H317,H336,H412
• Precautionary Statements: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378
• Hazard Classifications: Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2