05892970001
Roche
cOmplete™ ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail
Tablets supplied in foil blister packs.
Synonym(s):
protease inhibitor
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.28
Recommended Products
form
tablet
packaging
pkg of 30 tablets (individually packed in foil blister packs)
manufacturer/tradename
Roche
solubility
water: soluble
absorption
0.4 at 280 nm (1x solution)
storage temp.
2-8°C
General description
cOmplete ULTRA Tablets contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. As this tablet contains EDTA, metalloproteases are also inhibited. They are supplied as tablets in an EASYpack blister pack. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer. One cOmplete ULTRA, Mini Tablet is sufficient for a volume of 10 ml extraction solution.
Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. Metalloproteases, which are inhibited by EDTA, are fairly prevalent in, for example, bacteria.
You can easily inhibit both proteases and phosphatases at the same time by simply combining a cOmplete ULTRA, Mini, Protease Inhibitor Cocktail Tablet with a PhosSTOP Phosphatase Inhibitor Cocktail Tablet in the same 10 ml buffer.
Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. Metalloproteases, which are inhibited by EDTA, are fairly prevalent in, for example, bacteria.
You can easily inhibit both proteases and phosphatases at the same time by simply combining a cOmplete ULTRA, Mini, Protease Inhibitor Cocktail Tablet with a PhosSTOP Phosphatase Inhibitor Cocktail Tablet in the same 10 ml buffer.
Specificity
Inhibits serine proteases, cysteine proteases. Does not inhibit metalloproteases completely when divalent cations like Ca2+, Mg2+, or Mn2+ are present
Application
During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows highest inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine, cysteine, and metalloproteases. cOmplete ULTRA Tablets were developed for maximum efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
Roche ′s new resin for the purification of his-tagged proteins, cOmplete His-Tag Purification Resin, is compatible with this EDTA-containing tablet. Due to a new metal ion-binding-chelator chemistry in the cOmplete His-Tag Purification Resin, EDTA can be used during Immobilized Metal Ion Affinity Chromatography (IMAC).
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine, cysteine, and metalloproteases. cOmplete ULTRA Tablets were developed for maximum efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
Roche ′s new resin for the purification of his-tagged proteins, cOmplete His-Tag Purification Resin, is compatible with this EDTA-containing tablet. Due to a new metal ion-binding-chelator chemistry in the cOmplete His-Tag Purification Resin, EDTA can be used during Immobilized Metal Ion Affinity Chromatography (IMAC).
cOmplete™ ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail has been used as a component of radioimmunoprecipitation assay buffer (RIPA buffer). It has also been used to prevent protein degradation and coagulation in blood samples.
Features and Benefits
- Ultra efficient protection
- Convenient tablet format
- Suitable for IMAC purification
- Ensure laboratory safety
Components
Proprietary mixture of several protease inhibitors with broad inhibitory specificity.
Physical form
Tablets in foil blister packs. Each tablet is sufficient for a volume of 10 ml extraction solution.
Preparation Note
Working concentration: 1 tablet per 10 ml extraction solution.
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 10 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 10 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add cOmplete ULTRA Tablet to 5 ml double-distilled water in a PE or PP tube.
• Vortex 3 min or until the tablet is completely dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• Stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Working solution: Handling
Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One tablet is sufficient for the inhibition of proteolytic activity in 10 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 10 ml extraction buffer. The tablets can be added directly to the extraction medium.
Stock Solution (2x conc.)
Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add cOmplete ULTRA Tablet to 5 ml double-distilled water in a PE or PP tube.
• Vortex 3 min or until the tablet is completely dissolved.
• The stock solution is ready to use even if there are very fine particles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• Stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.
Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at -15 to -25 °C as well.
Reconstitution
Soluble in aqueous buffers, or add directly to extraction media.
For the stock solution, use water only.
For the stock solution, use water only.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
cOmplete is a trademark of Roche
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible, corrosive hazardous materials
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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