UFC8030

Amicon^® Ultra Centrifugal Filter, 30 kDa MWCO

sample volume 4 mL, regenerated cellulose membrane, MWCO 30 kDa

• Synonym(s): Centrifugal concentrator,30 kDa MWCO, 4 mL sample volume
• UNSPSC Code: 41104916
• eCl@ss: 36100101
• NACRES: NB.24
• material: polyethylene cap; polyethylene liner; polypropylene tube; regenerated cellulose membrane; regenerated cellulose support; styrene-butadiene housing
• pore size: 30 kDa MWCO
• L: 124 mm (4.88 in.)
• diam.: 17.3 mm
• filtration area: 3 cm²
• volume: 4 mL
• sterility: non-sterile

Properties

• material: polyethylene cap; polyethylene liner; polypropylene tube; regenerated cellulose membrane; regenerated cellulose support; styrene-butadiene housing
• Quality Level: 400
• sterility: non-sterile
• product line: Amicon^®
• manufacturer/tradename: Amicon^® Ultra
• parameter: 4 mL sample volume
• technique(s): protein extraction: suitable; protein purification: suitable
• L: 124 mm (4.88 in.)
• diam.: 17.3 mm
• filtration area: 3 cm²
• volume: 4 mL
• pore size: 30 kDa MWCO
• shipped in: ambient

Description

General description

Amicon^® Ultra 4 mL centrifugal devices with Ultracel^® 30,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Ovalbumin (45 kDa) at 1 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon^® Ultra 15 mL filters deliver the highest recovery with short spin times.

Application

• Ultrafiltration with 30,000 Dalton molecular weight cutoff Ultracel^® membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
• Concentration of dilute or pre-purified proteins from column eluents.
• Desalting, buffer exchange and protein dialysis.
• Size exclusion and protein fractionation.

Features and Benefits

• Ultracel^® regenerated cellulose membrane with 30 kDa NMWCO delivers retentate recovery of greater than 90%.
• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
• Heat-sealed membrane minimizes downstream extractables.
• 100% integrity tested for reliable performance.
• Convenient sample monitoring with translucent housing and volume gradations.
• Direct pipette sample access eliminates processing step to recover concentrate.
• High concentration factors of 80–100X.

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10-13 - German Storage Class 10 to 13