Product TMS-010 is a standard pH 2.5 solution of Alcian Blue that stains a number of substances, including chondrocytes, at this pH. The provided procedure is similar but not identical to the standard histology stain procedure found in most histology textbooks.
TMS-010
Alcian-Blue Staining Solution
Synonym(s):
Alcian blue 8G, Alcian blue PAS stain, Alcian blue stain
Sign Into View Organizational & Contract Pricing
Select a Size
100 ML
€118.00
€118.00
In StockDetails
All Photos(1)
Select a Size
Change View
100 ML
€118.00
About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
€118.00
In StockDetails
Recommended Products
technique(s)
cell based assay: suitable
cell culture | stem cell: suitable
cell differentiation: suitable
Quality Level
Related Categories
General description
Alcian blues, are a family of polyvalent basic dyes, of which, the Alcian blue 8G has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. Alcian blue if often used to identify Chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Osteobiology
Arthritis
Osteobiology
Arthritis
Quality
Solution is blue with no particulates present. Solution is suitable for endpoint cell culture assays.
Physical form
Product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
Product is presented as a 1% w/v Alcian Blue solution.
Storage and Stability
Store at room temperature for up to 4 months from date of receipt. If Alcian-Blue solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Flash Point(F)
102.2 °F - closed cup
Flash Point(C)
39 °C - closed cup
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.
Protocols
Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.
Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.
-
What is the composition of the differentiation for TMS 010 C procedure?
1 answer-
Helpful?
-
-
What is the concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product?
1 answer-
The concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product is not provided. However, it is a classical textbook formulation typically consisting of 1% Alcian Blue in 3% glacial acetic acid.
Helpful?
-
-
Is there a procedure available for using TMS-010, and in this context, would you anticipate the alcian blue to be present in the nuclei, cytoplasm, or the entire cell?
1 answer-
Depending on the preparation of Alcian Blue, the dye can be used to identify various substances. TMS-010 seems to have been formulated to stain chondrocytes, the cells that produce collagen. It's expected that the staining should be primarily in the cytoplasm. However, if the staining intensity is high, it might appear that the entire cell is stained.
Helpful?
-
-
Can you provide a protocol for a colorimetric assay that uses Alcian Blue to compare sulfated proteoglycan content in adherent cancer cell lines?
1 answer-
The Alcian-Blue Staining Solution (cat. no. TMS-010-C) is a cell culture product from the cell culture portfolio, intended for cell-based assays, cell differentiation, and stem cell culture. For example, to identify chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells (MSCs).
This product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
The Certificate of Analysis does not include any usage instructions and there is no separate product data sheet or user guide.
The following paper may be helpful for the staining of stem cells:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432525/Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
