MABN491
Anti-Brevican Antibody, clone N294A/6
clone N294A/6, from mouse
Synonym(s):
Brevican core protein, Brain-enriched hyaluronan-binding protein, BEHAB, Brevican
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
N294A/6, monoclonal
species reactivity
rat, human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BCAN(63827)
General description
Brevican core protein (BCAN), also known as Brain-enriched hyaluronan-binding protein (BEHAB) or Chondroitin sulfate proteoglycan 7 (CSPG7), may play a role in the terminally differentiating and the adult nervous system during postnatal development. It may stabilize interactions between hyaluronan (HA) and brain proteoglycans. Two isoforms exist, with isoform 1 (~99 kDa) being highly expressed from birth through 8 years of age and down-regulated by 20 years of age to low levels that are maintained in the normal adult cortex; and isoform 2 (~72 kDa) being expressed at uniformly low levels throughout development.
Immunogen
Recombinant protein corresponding to rat Brevican.
Application
Anti-Brevican, clone N294A/6 is an antibody targeting the Brevican protein, validated for use in WB & IHC.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Western Blot Analysis: 2 µg/mL from a representative lot detected Brevican in 10 µg of human brain tissue lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Brevican in rat midbrain, rat thalamus, and rat cerebellum tissues.
Western Blot Analysis: A representative lot detected Brevican in rat brain tissue lysate and in WT, but not in Brevican knockout mouse brain tissue lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Brevican in rat midbrain, rat thalamus, and rat cerebellum tissues.
Western Blot Analysis: A representative lot detected Brevican in rat brain tissue lysate and in WT, but not in Brevican knockout mouse brain tissue lysate.
Quality
Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected Brevican in 10 µg of rat brain tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected Brevican in 10 µg of rat brain tissue lysate.
Target description
~140 kDa observed. The calculated molecular weight of this protein is 96 kDa A second isoform is known to exist at 69 kDa This protein may be observed at a higher molecular weight due to glycosylation. A proteolyticaly cleaved fragment of Brevican may be observed at ~50 kDa in some tissue lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain tissue lysate
Rat brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ditte S Jonesco et al.
PloS one, 15(6), e0234632-e0234632 (2020-06-20)
Evidence indicate that the brain-specific protein, brevican, is proteolytically cleaved during neurodegeneration, hence positioning fragments of brevican as potential blood biomarkers of neurodegenerative diseases, such as dementia. We aimed to develop two assays capable of detecting the brevican N-terminal (N-Brev)
Moon Hang Kim et al.
Tissue engineering and regenerative medicine, 18(1), 165-178 (2020-09-18)
Chondroitin sulfate glycosaminoglycans (CS-GAGs) are the primary inhibitory GAGs for neuronal growth after central nervous system (CNS) injury. However, the inhibitory or permissive activity of CS-GAG subtypes is controversial and depends on the physiological needs of CNS tissues. In this
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