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MAB1585

Sigma-Aldrich

Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2

culture supernatant, clone 5A3.2, Chemicon®

Synonym(s):

Anti-ATITHS, Anti-BRN3A, Anti-Oct-T1, Anti-RDC-1, Anti-brn-3A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

5A3.2, monoclonal

species reactivity

chicken, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_006237.3

UniProt accession no.

Q01851

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... POU4F1(5457)

Specificity

Recognizes Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice.

Immunogen

Amino acids 186-224 of Brn-3a fused to the T7 gene 10 protein (Xiang et al., 1995).
Epitope: POU-domain protein

Application

Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2 detects level of Brn-3a & has been published & validated for use in IH & WB.
Immunohistochemistry: 1:5-1:125

Western blot: 1:50-1:100

Optimal working dilutions must be determined by end user.

Target description

46 kDa

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Embryonic retinal cells and support to mature retinal neurons.
Stanke, JJ; Fischer, AJ
Investigative Ophthalmology & Visual Science null
Combinatorial expression of Brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis.
Badea, TC; Williams, J; Smallwood, P; Shi, M; Motajo, O; Nathans, J
The Journal of Neuroscience null
Andy J Fischer et al.
The Journal of comparative neurology, 496(4), 479-494 (2006-03-31)
We recently identified large glucagon-expressing neurons that densely ramify neurites in the peripheral edge of the retina and regulate the proliferation of progenitors in the circumferential marginal zone (CMZ) of the postnatal chicken eye (Fischer et al. [2005] J Neurosci
Masahiro Miyake et al.
Nature communications, 6, 6689-6689 (2015-04-01)
Myopia can cause severe visual impairment. Here, we report a two-stage genome-wide association study for three myopia-related traits in 9,804 Japanese individuals, which was extended with trans-ethnic replication in 2,674 Chinese and 2,690 Caucasian individuals. We identify WNT7B as a
Sustained ocular hypertension induces dendritic degeneration of mouse retinal ganglion cells that depends on cell type and location.
Feng, L; Zhao, Y; Yoshida, M; Chen, H; Yang, JF; Kim, TS; Cang, J; Troy, JB; Liu, X
Investigative Ophthalmology & Visual Science null
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