The following changes have been implemented:
- Secretin has been added
- Assay Buffer has been changed to LE-ABGLP3 to enhance Insulin and Glucagon Detection
- The antibody pair for Insulin, IL-6, MCP-1, PP, and TNF-a has been replaced
HMH3-34K
MILLIPLEX® HUMAN METABOLIC HORMONE PANEL V3
Synonym(s):
human metabolic hormone immunoassay panel, luminex human metabolic hormone multiplex assay, millipore human metabolism multiplex kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.47
To order a Milliplex® kit, please search for your analyte of interest.
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species reactivity
human
Quality Level
manufacturer/tradename
Milliplex®
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex® xMAP®)
storage temp.
2-8°C
General description
Metabolic syndrome is a cluster of conditions that occur together; it includes increased blood pressure, elevated insulin levels, excess body fat around the waist and abnormal cholesterol levels.Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia and central obsesity- all of which are risk factors for atherosclerosis, coronary heart disease, type 2 diabetes, kidney disease, and even premature death.Adults with metabolic syndrome show a low-grade inflammation, whose link with obesity may be dysregulated adipocyte production of pro- and anti-inflammatory factors. Consequently, research done in this area covers multi-faceted fields of cytokines, acute phase proteins, diabetes, and obesity related hormones, as well as other cardiovascular disease biomarkers.MILLIPLEX® Human Metabolic Hormone Panel V3 is a 14-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma, tissue/cell lysates, and culture supernatant samples: Amylin Active or Total, C-Peptide, Ghrelin Active, GIP, GLP-1 Active or Total, Glucagon, IL-6, Insulin, Leptin, MCP-1/CCL2, PP, PYY, Secretin and TNFα.Note: Amylin Active and Total cannot be run together in the same assay. GLP-1 Active an GLP-1 Total cannot be run together in the same assay.
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Application
The Human Metabolic Hormone Panel V3, using the Luminex® xMAP technology, enables the simultaneous analysis of 14 protein biomarkers in human serum, plasma and tissue/lysate and culture supernatant samples.There are 16 biomarkers to choose from in this kit. However, this is a 14-plex kit since the following analytes cannot be run simultaneously: Amylin Active and Total cannot be run together in the same assay. GLP-1 Active and Total cannot be run together in the same assay.The following analytes require the addition of protease inhibitors to the serum or plasma samples (see sample collection and storage section in the protocol for details): GLP-1 (DPPIV inhibitor); Amylin (protease inhibitor cocktail); Glucagon (Aprotinin); Ghrelin (Active) (serine protease inhibitor). The protocol recommends serum or plasma samples be run neat. Tissue culture samples may be run neat or may require a dilution with an appropriate control medium. For optimal sensitivity, use an overnight (16 - 18 hour) incubation. Each well requires 25 uL of sample, standard or controlAnalytes available:Amylin (Total);C-Peptide;Ghrelin (Active);GIP;GLP-1 (Active);GLP-1 (Total);Glucagon;IL-6;Insulin;Leptin;MCP-1;PP;PYY;TNFα;Secretin;Amylin (Active)
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
96-well plate
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Could you provide me with the differences between the Human Metabolic Hormone Expanded Panel (HMHEMAG-34K) and the new Human Metabolic Hormone Panel V3 (HMH3-34K)?
1 answer-
Helpful?
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Would the BD P800 sample collection tubes be suitable for use in the Milliplex Human Metabolic Hormone Panel?
1 answer-
The BD P800 tubes contain inhibitors for GLP-1 active, Amylin, and Glucagon, but not for Active Ghrelin. If you also want to include active Ghrelin, you will need to add AEBSF.
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