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208750

Sigma-Aldrich

ALLN

InSolution, ≥95%, Inhibitor of calpain I, calpain II, cathepsin B, and cathepsin L

Synonym(s):

InSolution ALLN, Calpain Inhibitor I, LLNL, MG 101, Proteasome Inhibitor V

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About This Item

Empirical Formula (Hill Notation):
C20H37N3O4
CAS Number:
110044-82-1
Molecular Weight:
383.53
MDL number:
MFCD00065505
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

100

Assay

≥95% (HPLC)

form

solution

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

clear colorless

shipped in

wet ice

storage temp.

−20°C

InChI

1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1

InChI key

FMYKJLXRRQTBOR-BZSNNMDCSA-N

General description

A 10 mM solution of ALLN (Cat. No. 208719) in anhydrous DMSO. Inhibitor of calpain I (Ki = 190 nM), calpain II (Ki = 220 nM), cathepsin B (Ki = 150 nM), and cathepsin L (Ki = 0.5 nM). Inhibits neutral cysteine proteases and proteasome (Ki = 6 µM). Protects against neuronal damage caused by hypoxia and ischemia. Inhibits apoptosis in thymocytes and metamyelocytes. Inhibits cell cycle progression at the G1/S border and metaphase/anaphase in CHO cells by inhibiting cyclin B degradation. Also prevents nitric oxide production by activated macrophages by interfering with transcription of the inducible nitric oxide synthase gene. Inhibits the proteolysis of IkB-α and IkB-β by the ubiquitin-proteasome complex.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
calpain 1
Product does not compete with ATP.
Reversible: no
Target Ki: 190 nM, 220 nM, 150 nM, 500 pM, against calpain I, calpain II, cathepsin B, and cathepsin L, respectively

Packaging

Packaged under inert gas

Warning

Toxicity: Flammable (J)

Physical form

A 10 mM (5 mg/1.30 ml) solution of ALLN (Cat. No. 208719) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Milligan, S.A., et al. 1996. Arch. Biochem. Biophys.335, 388.
Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721.
Squier, M.K., et al. 1994. J. Cell Physiol.159, 229.
Rami, J., and Kreiglstein, J. 1993. Brain Res.609, 67.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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