WNK lysine deficient protein kinase 3, serine/threonine-protein kinase WNK3, Protein kinase with no lysine 3, Protein kinase lysine-deficient 3, protein kinase, lysine deficient 3
WNK3 is a serine-threonine protein kinase and expressed throughout the nephron, but most commonly at intercellular junctions. In its active state, it is a promoter of NKCC2 and NCC activity. The opposite is true of WNK3 when in an inactive state. WNK3 changes the expression of these transporters in the membrane and is thought to be a regulator of blood pressure along with other members of the WNK family of protein kinases. In recent research, WNK3 has also been linked to regulating signaling cascades involved in tumorigenesis.
Specificity
Other homologies: Rat (74% sequence homology).
Immunogen
GST-tagged recombinant protein corresponding to human WNK3.
Application
Research Category Signaling
Research Sub Category Developmental Signaling
This WNK3 antibody is validated for use in WB for the detection of the WNK3 protein.
Quality
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected WNK3 on 10 µg of HEK293 cell lysate.
Target description
~220 kDa observed. Uncharacterized bands appear at ~52 and 60 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control HEK293 cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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