Interleukin-4 (IL-4) is a cytokine that is a key regulator in humoral and adaptive immunity. IL-4 induces differentiation of naive helper T-cells (Th0 cells) to Th2 cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Further, IL-4 controls the production of IgE, expands IL-4 producing T-cell subsets and stabilizes effector cell functions. IL-4 has great therapeutic potential due to its role in the development of allergic inflammatory responses. IL-4 also has shown to have promise in drug targeting for cancer.
The SMC® Mouse IL-4 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.
Application
Research Category Inflammation & Immunology
Research Sub Category Cytokines & Cytokine Receptors
The Single Molecule Counting (SMC®) Mouse IL-4 Discovery Immunoassay has unparalleled performance in quantifying IL-4 in mouse serum.
Used to detect/quantify:IL-4
Components
Assay Buffer Mouse IL-4 Coating Antibody Assay Plate 10X PBS Blocking Buffer Standard Diluent Mouse IL-4 Detection Antibody Mouse IL-4 Standard SMC® Mouse IL-4 Immunoassay Kit Instructions 10X Wash Buffer Buffer C Elution Buffer B
Analysis Note
Low-end CV% Range: 0-20%|Low-end CV% Average: 4%
Legal Information
SMC is a registered trademark of Singulex
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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