Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
Xylene Cyanol FF is used as a:
histological stain and as tracker dye in DNA sequencing and electrophoresis[1][2]
Trends in biochemical sciences, 20(5), 202-203 (1995-05-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methods-reagents, available on the Internet. This month's column describes how to make an electrophoresis system from common household items. For details on how to partake
Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells
<BIG>Thiel WH, et al.</BIG>
Methods in Molecular Biology, 187-199 (2015)
Design and self-assembly of siRNA-functionalized RNA nanoparticles for use in automated nanomedicine
RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive
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