SRP3171
TGF-beta 3-E. coli human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
Transforming Growth Factor-beta3
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About This Item
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biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.05 ng/mL ED50
mol wt
25.0 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TGFB3(7043)
General description
The gene TGF-β3 (transforming growth factor β 3) is mapped to human chromosome 14q24.3. The three mammalian isoforms of TGF-β, TGF-β1, β2, β3 elicit similar biological responses. TGF-β proteins are made as precursor molecules which are cut to generate a mature carboxy-terminal region of 110-140 amino acids. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-β isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. Recombinant TGF-β3 is a 25.0kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
Application
TGF-β3 (transforming growth factor β 3) human has been used in the media for the chondrogenic differentiation of the stromal cells. It has been used in the culture medium to allow attachment of primary human dermal fibroblasts to the scaffolds.
Biochem/physiol Actions
The three mammalian isoforms of TGF-β (transforming growth factor β), TGF-β1, β2, β3, are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and wound healing. TGF-β3 is the predominant TGF-β in the testis and controls the blood-testis barrier activity. Polymorphisms in this gene might be associated with male infertility. Heterozygous mutation in the gene at position Arg300 might be associated with increased TGF-β signaling, leading to a disorder with phenotypic overlap with Marfan syndrome and Loeys-Dietz syndrome. TGF-β3 is involved in the mesenchymal stem cells (MSCs)-mediated inhibition of fibroblast proliferation and decreases skin fibrosis.
Sequence
ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS
Physical form
Lyophilized from 0.1% TFA
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 °C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 °C to -80 °C.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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