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S4945

Sigma-Aldrich

Anti-SynCAM antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-IGSF4, Anti-Necl-2, Anti-RAI75, Anti-SgIGSF, Anti-Tslc1

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

mouse, rat

technique(s)

immunohistochemistry: suitable using suitable for IHC on rat brain perfused with 4% paraformaldehyde
microarray: suitable
western blot: 0.5-1.0 μg/mL using mouse brain and rat brain extract (S1 fraction)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Cadm1(54725)
rat ... Cadm1(363058)

General description

Anti-SynCAM is developed in rabbit using a synthetic peptide corresponding to amino acids located at the C-terminus of mouse SynCAM1 conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Synaptic cell adhesion molecule (SynCAM) is a brain-specific protein which has an immunoglobulin domain. It binds to intracellular PDZ-domain protein. SynCAMs have also been called nectin-like molecules (Necls). SynCAM1 is also known as Necl-2/ IGSF4/ RA175/ SgIGSF/ TSLC1. SynCAM contains an N-terminal signal peptide, three extracellular Ig domains, a single transmembrane region and a short C-terminal cytoplasmic domain. It is expressed only in brain and is localized to synapses.
SynCAM proteins consist of a C-terminal cytoplasmic domain, an N-terminal signaling domain and three extracellular immunoglobulin domains.. Anti-SynCAM antibody is specific for SynCAM in mouse and rats. The SynCAM1 immunizing peptide (mouse, amino acids 431-445) specifically inhibits the staining of the SynCAM band in immunoblotting.

Immunogen

This immunogen sequence is identical in human SynCAM1, highly conserved (90%) in the splice variant SynCAM2 and displays limited homology (55%) to SynCAM3.
synthetic peptide corresponding to amino acids 431-445 located at the C-terminus of mouse SynCAM1 conjugated to KLH.

Application

  • Anti-SynCAM antibody produced in rabbit has been used in:
  • immunoprecipitation
  • immunoblotting
  • immunohistochemistry
  • immunostaining
  • immunofluorescence

Biochem/physiol Actions

SynCAM is a neurological cell-adhesion protein that regulates the formation of synapses. Expression of synaptic cell adhesion molecule (SynCAM) in non-neuronal cells induces synaptic formation and affects synaptic function in hippocampal neurons. It functions as a homophilic cell adhesion molecule at the synapse. SynCAM is believed to interact with PDZ-domain synaptic proteins CASK and syntenin via its cytoplasmic tail.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fuka Takeuchi et al.
Cells, tissues, organs, 213(4), 326-337 (2023-10-31)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) first infects the host nasal mucosa, where the viral spike protein binds to angiotensin-converting enzyme 2 (ACE2) on the mucosal cells. This study aimed at searching host cell surface molecules that could contribute
Johan Botling et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 19(1), 194-204 (2012-10-04)
Global gene expression profiling has been widely used in lung cancer research to identify clinically relevant molecular subtypes as well as to predict prognosis and therapy response. So far, the value of these multigene signatures in clinical practice is unclear
Markus Missler
Trends in neurosciences, 26(4), 176-178 (2003-04-12)
A growing number of candidate genes has been implicated in linking the two sides of a synapse but definitive proof of a specific role for many of them is still scarce. Exploiting the vast amount of sequence data, a novel
Ying Gao et al.
PloS one, 8(5), e64316-e64316 (2013-06-07)
Nectin-like molecule-2 (Necl-2), a junction molecule, is exclusively expressed by spermatogenic cells. It mediates homophilic interaction between germ cells and heterophilic interaction between Sertoli and germ cells. Knockout studies have shown that loss of Necl-2 causes male infertility, suggesting Necl-2-based
SynCAM, a synaptic adhesion molecule that drives synapse assembly
Biederer T, et al.
Science, 297(5586), 1525-1531 (2002)

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