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N0163

Sigma-Aldrich

Nitrate Reductase from Arabidopsis thaliana

vial of ≥0.5 unit

Synonym(s):

NADH:nitrate oxidoreductase

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About This Item

Enzyme Commission number:
1.7.1.1 (BRENDA, IUBMB)
MDL number:
MFCD00131725
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in Pichia pastoris

Quality Level

200

form

lyophilized powder

packaging

vial of ≥0.5 unit

shipped in

wet ice

storage temp.

−20°C

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Application

Catalyzes the NADH-dependent reduction of nitrate to nitrite.
Nitrate reductase from Arabidopsis thaliana has been used in a study to assess the amino acid sequence of chicken hepatic sulfite oxidase.

Biochem/physiol Actions

Nitrate reductase activity is induced in Arabidopsis thaliana plants by sumoylation via the E3 ligase activity of AtSIZ1.

Unit Definition

One unit will reduce 1.0 micromole of nitrate to nitrite per min in a NADH system at pH 7.5 at 30 deg C.

Physical form

Supplied as a lyophilized powder containing 50 mM MOPS, pH 7.0, 0.1 mM EDTA and a proprietary sugar

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Precautionary Statements

P261 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN4623
SLCG2294
SLCM7879
SLCK8803
SLCC9947

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Conserved domains in molybdenum hydroxylases. The amino acid sequence of chicken hepatic sulfite oxidase
Neame, P. and M. Barber
The Journal of Biological Chemistry, 164, 20894-20901 (1989)
Arabidopsis nitrate reductase activity is stimulated by the E3 SUMO ligase AtSIZ1
Park, BS
Nature Communications, 19, 400-400 (2011)
Tatsuya Fujii et al.
Bioscience, biotechnology, and biochemistry, 72(2), 412-420 (2008-02-08)
Fusarium oxysporum JCM11502 expresses a denitrifying (nitrate (NO(3)(-))-respiring) mechanism and can thrive under oxygen (O(2)) limitation. The fungus reduces NO(3)(-) to nitrite at the initial step of denitrification. In this study, we cloned the gene coding NADH-NO(3)(-) reductase (NADH-Nar) (niaD)
Stefania Astolfi et al.
Journal of plant physiology, 161(7), 795-802 (2004-08-18)
The interactions between sulphur nutrition and Cd exposure were investigated in maize (Zea mays L.) plants. Plants were grown for 12 days in nutrient solution with or without sulphate. Half of the plants of each treatment were then supplied with
D Pattanayak et al.
Molecular cell biology research communications : MCBRC, 1(3), 237-240 (1999-11-30)
Chemical modification of purified nitrate reductase (NR) from sunflower leaves by white light-irradiated rose bengal was studied. NADH:NR activity was inhibited by light-activated rose bengal in both a concentration- and time-dependent manner. MV:NR activity was less sensitive to inhibition than
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