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G6880

Sigma-Aldrich

α-Glycerophosphate Dehydrogenase from rabbit muscle

Type X, lyophilized powder, ≥100 units/mg protein

Synonym(s):

sn-Glycerol-3-phosphate Dehydrogenase from rabbit muscle, sn-Glycerol-3-phosphate:NAD+ 2-oxidoreductase

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About This Item

CAS Number:
9075-65-4
Enzyme Commission number:
1.1.1.8 (BRENDA, IUBMB)
EC Number:
232-982-3
MDL number:
MFCD00130487
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

type

Type X

Quality Level

200

form

lyophilized powder

specific activity

≥100 units/mg protein

composition

Protein, ≥75%

foreign activity

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
Triosephosphate isomerase ≤0.02%

shipped in

wet ice

storage temp.

−20°C

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Application

α-glycerophosphate dehydrogenase was used in 2-deoxy-ribose 5-phosphate aldolase (DERA) cleavage (retroaldol) assay.[1]

Biochem/physiol Actions

α-glycerophosphate dehydrogenase catalyzes the conversion of dihydroxyacetone to glycerol phosphate[2].

Unit Definition

One unit will convert 1.0 μmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.

Physical form

Lyophilized sulfate-free powder containing buffer salts as citrate and EDTA

Analysis Note

Protein determined by biuret

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000429784
0000429784
0000384630
0000374399
0000338226

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Kui-Yi Xing et al.
Investigative ophthalmology & visual science, 51(12), 6598-6604 (2010-07-09)
To investigate the effect of age on the key oxidation repair enzymes of the thioltransferase (TTase) and thioredoxin (TRx) systems in the human lens. Twenty-three normal human lenses (donor ages, 19-77 years) were grouped into second, third, fifth, sixth, and
Zhong-peng Guo et al.
Journal of industrial microbiology & biotechnology, 38(8), 935-943 (2010-09-09)
The GPD2 gene, encoding NAD(+)-dependent glycerol-3-phosphate dehydrogenase in an industrial ethanol-producing strain of Saccharomyces cerevisiae, was deleted. And then, either the non-phosphorylating NADP(+)-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN) from Bacillus cereus, or the NADP(+)-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Kluyveromyces lactis, was expressed
Branka Stirn Kranjc et al.
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 247(11), 1505-1515 (2009-07-18)
To compare the organization of human and rat ocular medial recti muscles (MR). The cryosections of human and rat MR were processed for myofibrillar ATPase (mATPase), succinate dehydrogenase and glycerol-3-phosphate dehydrogenase. To reveal myosin heavy chain (MyHC) isoforms, specific monoclonal
B S Muhlhausler et al.
Endocrinology, 150(9), 4287-4294 (2009-06-13)
Exposure to maternal overnutrition increases the expression of peroxisome proliferator-activated receptor-gamma (PPARgamma) in adipose tissue before birth, and it has been proposed that the precocial activation of PPARgamma target genes may lead to increased fat deposition in postnatal life. In
Carmen E Perrone et al.
Metabolism: clinical and experimental, 59(7), 1000-1011 (2010-01-05)
Methionine restriction increases life span in rats and mice and reduces age-related accretion of adipose tissue in Fischer 344 rats. Recent reports have shown that adipose tissue mitochondrial content and function are associated with adiposity; therefore, the expression of genes
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