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Key Documents

EoL-1 cell

94042252, human blood, Lymphoblast

Synonym(s):

AML-EOL-1 Cells, EoL1 Cells, Eosinophilic Leukemia Cell Line

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About This Item

UNSPSC Code:
41106514

product name

EoL-1 cell, 94042252

biological source

human blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human eosinophilic leukaemia

Cell Line Description

Differentiated by N-butylate treatment.

Application

EoL-1 cell line has been used to study the role of AGS67E, an antibody-drug conjugate that targets cluster of differentiation-37 (CD37) for the treatment of B/T-cell malignancies. It has also been used to study the role of anti-vascular cell adhesion molecule-1 (VCAM-1) monoclonal antibody in inhibiting asthma in a murine asthma model.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 12,13
D16S539: 8,10
D5S818: 11
D7S820: 10,13
THO1: 9.3
TPOX: 8
vWA: 16,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Eol-1 cells grow very slowly and may take up to 5 days to reach a suitable level to subculture. It is best that these cells are left to grow and not agitated more than necessary.The cells actually grow as large clusters. They are beginning to reach confluence when the cells in the centre of each cluster start to look unhealthy. The change in media colour should also be an indication. Eol-1 cells prefer slightly acidic conditions and so may resuscitate better from frozen if started up in 15% foetal bovine serum.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

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