C9281

Cholesterol Esterase from Pseudomonas fluorescens

lyophilized powder, ≥10,000 units/g protein

• Synonym(s): CE, bile salt-stimulated lipase, cholesteryl ester hydrolase, pancreatic cholesterol esterase, Sterol-ester acylhydrolase
• CAS Number: 9026-00-0
• Enzyme Commission number: 3.1.1.13 (BRENDA, IUBMB)
• EC Number: 232-808-6
• MDL number: MFCD00071052
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Pseudomonas fluorescens
• Quality Level: 200
• Assay: 10-30% (TCA-Biuret)
• form: lyophilized powder
• specific activity: ≥10,000 units/g protein
• mol wt: ~129 kDa
• composition: Protein, ~20%
• storage condition: under inert gas (argon)
• technique(s): cell based assay: suitable
• color: tan to brown
• pH: 7-9
• solubility: 0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL
• storage temp.: −20°C

Description

General description

Research area: Cell Signaling

Cholesterol Esterase (CE) is a glycoprotein that can be isolated from fungal species such as Candida cylindracea and Pseudomonas fluorescens. It is classified as a member of the lipase/esterase family and functions as a homo-dimeric protein. CE is produced in the pancreas and is released in an active form upon stimulation by Cholecystokinin (CCK).

Application

Cholesterol esterase from Pseudomonas fluorescens has been used in:

• in cholesterol esterase assay to quantify total cholesterol from human blood serum samples
• a study to investigate the nondenaturing protein electro transfer of the esterase activity of lipolytic preparations
• an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase
• for the modification of human plasma low-density-lipoprotein (LDL) to induce endothelial cell (EC) dysfunction and monocyte (MC) adhesion in the branched tissue-engineered blood vessels (TEBVs)
• to hydrolyze native cholesterol ester (CE) during ​filipin staining for detection of CE within the retinal frozen sections

This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. This activity of CE plays a vital role in steroid biosynthesis or de novo steroidogenesis. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis..

Other Notes

Contains potassium phosphate and TRITON® X-100.

Unit Definition

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

Analysis Note

Protein determined by biuret.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves