SCR066
Quantitative Alkaline Phosphatase ES Characterization Kit
This Quantitative Alkaline Phosphatase Embryonic Stem (ES) Characterization Kit easily quantifies the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay.
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.81
Recommended Products
Quality Level
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type: human embryonic stem cell(s)
sample type cardiac stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
General description
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and are capable of unlimited, undifferentiated proliferation in vitro under appropriate cell culture conditions. Undifferentiated embryonic stem cells are characterized by high expression levels of stage-specific embryonic antigens (SSEA-1, 3 and 4), tumor rejection antigens (TRA-1-60 and TRA-1-81), OCT-4 and alkaline phosphatase. These undifferentiated markers are downregulated upon spontaneous or induced differentiation.
Alkaline phosphatase (ALP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. The enzyme is present within all tissues of the body but is elevated in cells of the liver, kidney, bone, placenta, embryo and under specific disease states. Under alkaline conditions (pH>10), ALP can catalyze the hydrolysis of p-nitrophenylphosphate (p-NPP) into phosphate and p-nitrophenol, a yellow colored by-product of the catalytic reaction. The amount of p-nitrophenol produced is proportional to the amount of alkaline phosphatase present within the reaction. The amount of ALP can thus be reliably quantified by reading the amount of p-nitrophenol amassed after the catalytic reaction at 405 nm on a spectrophotometer.
Alkaline phosphatase (ALP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. The enzyme is present within all tissues of the body but is elevated in cells of the liver, kidney, bone, placenta, embryo and under specific disease states. Under alkaline conditions (pH>10), ALP can catalyze the hydrolysis of p-nitrophenylphosphate (p-NPP) into phosphate and p-nitrophenol, a yellow colored by-product of the catalytic reaction. The amount of p-nitrophenol produced is proportional to the amount of alkaline phosphatase present within the reaction. The amount of ALP can thus be reliably quantified by reading the amount of p-nitrophenol amassed after the catalytic reaction at 405 nm on a spectrophotometer.
Millipore’s Quantitative Alkaline Phosphatase ES Characterization Kit allows researchers to easily quantify the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay. This kit can be used in conjunction with Millipore’s Alkaline Phosphatase Detection kit (Cat. No. SCR004) to fully characterize the levels of alkaline phosphatase present within an embryonic stem cell culture.
Application
Research Category
Stem Cell Research
Stem Cell Research
This Quantitative Alkaline Phosphatase Embryonic Stem (ES) Characterization Kit easily quantifies the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay.
Components
p-NPP Substrate Concentrate (50X): (Part No. ES008-200UL) 200 μL
p-NPP Buffer: (Part No. ES011-10ML) 10 mL
Reaction Stop Solution: (Part No.CS200653) 5 mL
1X Wash Solution: (Part No. CS200652) 2 X 125 mL
Recombinant Alkaline Phosphatase Standard: (Part No. CS200654) 0.5 μg (10 μg/mL)
p-NPP Buffer: (Part No. ES011-10ML) 10 mL
Reaction Stop Solution: (Part No.CS200653) 5 mL
1X Wash Solution: (Part No. CS200652) 2 X 125 mL
Recombinant Alkaline Phosphatase Standard: (Part No. CS200654) 0.5 μg (10 μg/mL)
Storage and Stability
All reagents are to be stored at 2∘ to 8∘C until expiration date. p-NPP substrate concentrate and p-NPP buffer are light sensitive and should not be exposed to light. Reaction stop solution contains 1N sodium hydroxide and is corrosive. Avoid skin contact and inhalation.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
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Fei Deng et al.
Molecular vision, 18, 2871-2881 (2012-12-13)
This study aimed to develop a feasible and efficient method for generating embryonic stem cell (ESC)-like induced pluripotent stem (iPS) cells from human Tenon's capsule fibroblasts (HTFs) through the expression of a defined set of transcription factors, which will have
Ho-Chang Jeong et al.
Stem cells (Dayton, Ohio), 35(9), 2037-2049 (2017-05-26)
Basic fibroblast growth factor (bFGF) supplementation is critical to maintain the pluripotency of human pluripotent stem cells (hPSCs) through activation of PI3K/AKT, rather than MEK/ERK pathway. Thus, elaborate molecular mechanisms that preserve PI3K/AKT signaling upon bFGF stimulation may exist in
Jian Fang Liu et al.
Tissue engineering. Part A, 17(17-18), 2343-2357 (2011-05-10)
Differentiation of embryoid bodies (EBs) into particular cell lineages has been extensively studied. There is an increasing interest in the effect of soft hydrogel scaffolds on the behavior of EBs, such as the initial adhesion, dynamic morphology change, and differentiation.
Madalina Icriverzi et al.
Materials (Basel, Switzerland), 12(20) (2019-10-23)
The potential of mesenchymal stem cells (MSCs) for implantology and cell-based therapy represents one of the major ongoing research subjects within the last decades. In bone regeneration applications, the various environmental factors including bioactive compounds such as growth factors, chemicals
Narimon Honarpour et al.
Molecular & cellular proteomics : MCP, 13(3), 780-791 (2014-01-07)
The programmed formation of specific tissues from embryonic stem cells is a major goal of regenerative medicine. To identify points of intervention in cardiac tissue formation, we performed an siRNA screen in murine embryonic stem cells to identify ubiquitin system
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