The panel is capable of measuring hormones in mouse serum and plasma. Sample data is provided for reference. Additionally, spike recovery and dilutional linearity tests were conducted using mouse samples in the kit, yielding a mean recovery of 98% and a mean linearity of 109%.
MSHMAG-21K
MILLIPLEX® Multi-Species Hormone Magnetic Bead Panel
The Multi-Species Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 steroid/thyroid biomarkers in human serum, plasma and cell culture samples.
Synonym(s):
luminex multiple species thyroid reproductive stress endocrine metabolic hormones multiplex assay, millipore multiple species metabolism hormones multiplex kit, multi species hormone immunoassay panel
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
To order a Milliplex® kit, please search for your analyte of interest.
Recommended Products
Quality Level
species reactivity
human, canine
manufacturer/tradename
Milliplex®
assay range
accuracy: 105%
(Canine Samples: T3)
accuracy: 80%
(Canine Samples: Estradiol)
accuracy: 85%
(Human Samples: Progesterone)
accuracy: 87%
(Canine Samples: T4)
sensitivity: 0.01 ng/mL
(MinDC+2SD; Estradiol)
sensitivity: 0.12 ng/mL
(MinDC+2SD; T3)
sensitivity: 0.25 ng/mL
(MinDC+2SD; Progesterone)
sensitivity: 0.33 ng/mL
(MinDC+2SD; Cortisol)
sensitivity: 0.51 ng/mL
(MinDC+2SD; T4)
standard curve range: 0.014-10 ng/mL
(Estradiol)
standard curve range: 0.07-50 ng/mL
(T3)
standard curve range: 0.14-100 ng/mL
(Progesterone)
standard curve range: 0.27-200 ng/mL
(Cortisol)
standard curve range: 0.34-250 ng/mL
(T4)
inter-assay cv: <10%
intra-assay cv: <10%
(Cortisol)
inter-assay cv: <15%
intra-assay cv: <10%
(Estradiol)
inter-assay cv: <15%
intra-assay cv: <10%
(Progesterone)
inter-assay cv: <15%
intra-assay cv: <10%
(T3)
inter-assay cv: <15%
intra-assay cv: <10%
(T4)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
ambient
General description
Steroid and thyroid hormones are small molecule endocrine hormones secreted from adrenal glands (cortisol), ovaries (estradiol and progesterone), and the thyroid (T3 and T4). These hormones play key roles in the regulation of energy balance, mental and physical development, sex organ development, reproduction, and pregnancy. These hormones play a role in the patho-physiology of many diseases such as thyroid-related diseases (Grave’s disease and hyper/hypothyroidism), steroid-related diseases (Cushing’s syndrome) and stress related diseases. Endocrine disrupting chemincals (EDC) can impact normal functioning of steroid and thyroid hormones.
The MILLIPLEX® Multi-Species Hormone Panel is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Cortisol, Estradiol, Progesterone, T3 and T4. This multi-species kit may be used to detect these analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. The kit has been verified with human and canine samples. The amount of cross-reactivity to other species has been confirmed, but not tested to the same level of verification as was used for human and canine. All analytes in this kit are run in a competitive assay format. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
The MILLIPLEX® Multi-Species Hormone Panel is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Cortisol, Estradiol, Progesterone, T3 and T4. This multi-species kit may be used to detect these analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. The kit has been verified with human and canine samples. The amount of cross-reactivity to other species has been confirmed, but not tested to the same level of verification as was used for human and canine. All analytes in this kit are run in a competitive assay format. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
Specificity
Approximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.
This multi-species kit may be used to detect these 5 analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was fully validated with human and canine samples only and the amount of cross-reactivity to other species tested has not been determined.
This multi-species kit may be used to detect these 5 analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was fully validated with human and canine samples only and the amount of cross-reactivity to other species tested has not been determined.
Cross Reactivty
Approximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.
Approximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.
Application
- Analytes: Cortisol, Estradiol, Progesterone, T3, T4
- NOTE: This multi-species kit may be used to detect these analytes in human, dog, rat, mouse, cynomolgus monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was validated with human and canine samples only and the amount of cross-reactivity to other species tested, has not been determined.
- NOTE: This assay requires an extraction step for serum and plasma samples (instructions are provided in the kit protocol).
- NOTE: This is a competitive immunoassay
- Recommended Sample Type: Serum, plasma, or tissue/cell culture supernatants or lysates from multiple species
- Recommended Sample Dilution: 25 μL per well of undiluted extracted serum or plasma. Tissue/cell culture samples may be run neat or diluted in an appropriate control media.
- Assay Run Time: Overnight (16-20 hours) at 2-8°C.
- Research Category: Metabolism
- Research Category: Endocrine
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
96 well plate
Everything you need in a single kit.
Linkage
Replaces: STTHMAG-21K
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Other Notes
Please contact Technical Service for linearity of dilution.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Christian K Garcia et al.
PloS one, 17(10), e0275715-e0275715 (2022-10-14)
The capability of male mice to exercise in hot environments without succumbing to exertional heat stroke (EHS) is markedly blunted compared to females. Epidemiological evidence in humans and other mammals also suggests some degree of greater vulnerability to heat stroke
Yepei Fu et al.
Experimental and therapeutic medicine, 20(6), 244-244 (2020-11-13)
Kidney-yang deficiency syndrome (KYDS) infected with the influenza virus is a suitable model to imitate a population at high-risk to influenza infection with a high rate of morbidity and mortality. However, the specific molecular mechanisms underlying this disease remain unclear.
Gabriela Leite et al.
Menopause (New York, N.Y.), 29(3), 264-275 (2022-02-26)
Hormone therapy (HT) is used to treat menopause-related conditions and symptoms. The small intestine plays key roles in metabolic and endocrine function, but the effects of HT on the small intestinal microbiome are unknown. Here, we characterize duodenal microbiome differences
Related Content
The newer xMAP® Sheath Fluid PLUS formulation contains 3 modes of microbial action to eliminate potential environmental microbial resistance. See how the preparations compare in this performance comparison of the previous xMAP® Sheath Fluid to the new xMAP® Sheath Fluid PLUS formulation in MILLIPLEX® multiplex assays.
The newer xMAP® Sheath Fluid PLUS formulation contains 3 modes of microbial action to eliminate potential environmental microbial resistance. See how the preparations compare in this performance comparison of the previous xMAP® Sheath Fluid to the new xMAP® Sheath Fluid PLUS formulation in MILLIPLEX® multiplex assays.
The newer xMAP® Sheath Fluid PLUS formulation contains 3 modes of microbial action to eliminate potential environmental microbial resistance. See how the preparations compare in this performance comparison of the previous xMAP® Sheath Fluid to the new xMAP® Sheath Fluid PLUS formulation in MILLIPLEX® multiplex assays.
The newer xMAP® Sheath Fluid PLUS formulation contains 3 modes of microbial action to eliminate potential environmental microbial resistance. See how the preparations compare in this performance comparison of the previous xMAP® Sheath Fluid to the new xMAP® Sheath Fluid PLUS formulation in MILLIPLEX® multiplex assays.
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Can the Milliplex Multi-Species Hormone Panel (MSHMAG) detect the hormones in mouse serum/plasma?
1 answer-
Helpful?
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When purchasing a magnet for Biotek 405 TS or LS washers, which magnet should be used?
1 answer-
We use the following flat magnets for our washers: 7103016 for 96-well and 7103017 for 384-well. To set up the instrument for the magnet, go into the setup menu, navigate to the MAGHT option, and select YES for magnet installed. This will change the offsets for the z-height to account for the magnet being present.
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Have any other solvent extraction methods besides acetonitrile been tested on the multi-species hormone (MSHMAG) panel?
1 answer-
The kit protocol includes an acetonitrile extraction method, which has been tested and validated for use with this kit.
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Is it possible to combine MSHMAG-21K and RSHMAG-69K?
1 answer-
The formats of the two assays, in addition to Serum Matrix and sample extraction, are different. Mixing detection antibodies is not straightforward and may impact the performance of the assay.
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why do we have to extract samples of serum and plasma while using MSHMAG-21K?
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This kit exhibits strong matrix effects when utilized with serum and plasma samples. In other words, serum and plasma contain substances that interfere with the assay, so the extraction is required in order to remove them
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Does the Multi-Species Hormone kit recognize forms of testosterone, and specifically, does it recognize DHT?
1 answer-
The Testosterone capture antibody displays 100% reactivity to Testosterone and 16% cross-reactivity to 5-alpha-Dehydrotestosterone.
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