MABN713

Anti-CA9 Antibody, clone 2D3

ascites fluid, clone 2D3, from mouse

• Synonym(s): Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, CA-IX, Membrane antigen MN, Renal cell carcinoma-associated antigen G250, RCC-associated antigen G250, pMW1, CA9
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 2D3, monoclonal
• species reactivity: human
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1
• UniProt accession no.: Q16790
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CA9(768)

Description

General description

CA9 (Carbonic anhydrase 9) is a single-pass type I membrane protein belonging to the alpha-carbonic anhydrase family. CA9 may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. CA9 is primarily expressed in carcinoma cell lines and is found on the surface microvilli and in the nucleus.

Immunogen

Purified recombinant fragment of human CA9 expressed in E. Coli.

Application

Anti-CA9 Antibody, clone 2D3 is a highly specific mouse monoclonal antibody, that targets Carbonic Anhydrase & has been tested in western blotting, IHC & Flow Cytometry.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CA9 in human stomach and colon tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CA9 in NTERA-2 cells.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blotting in HeLa and A549 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CA9 in HeLa and A549 cell lysates.

Target description

~50 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa and A549 cell lysates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable