MAB4234
Anti-Wilms′ Tumor Antibody, NT, clone 6F-H2
clone 6F-H2, Chemicon®, from mouse
Synonym(s):
WT1
About This Item
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6F-H2, monoclonal
species reactivity
human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... WT1(7490)
Related Categories
General description
Specificity
Immunogen
Application
Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in the CRE-binding protein/CBP immunoprecipitate obtained from the lysate of a T-SV40 immortalized human glomerular epithelial cell (HGEC) line (Drossopoulou, G.I., et al. (2009). Am. J. Physiol. Renal Physiol. 297(3):F594-F603).
Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in lysates from mouse E15.5 embryonic kidney and human melanoma cell lines A375, SK-MEL-28, and WM-266-4 (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunocytochemistry Analysis: A representative lot immunostained the nucleus of methanol-fixed human melanoma A375 cells by fluorescent immunocytochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunofluorescence Analysis: A representative lot immunostained the PCNA-positive nuclei of proliferating cells in formalin-fixed, paraffin-embedded human melanoma tissue sections by fluorescent immunohistochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunohistochemistry Analysis: A representative lot immunostained glomeruli in formalin-fixed, paraffin-embedded normal human kidney and Wilms′ tumor sections (Wagner, N., et al. (2008). Pediatr. Nephrol. 23(9):1445-1453).
Immunohistochemistry Analysis: A representative lot detected vascular WT1 expression in 95% of 113 paraffin-embedded tumour tissues of various types. In most cases, nuclear WT1 staining of endothelial cells was seen (Wagner, N., et al. (2008). Oncogene. 27(26):3662-3672).
Immunohistochemistry Analysis: A representative lot immunostained the nucleus of perifollicular fibroblasts at the hair follicle in formalin-fixed, paraffin-embedded normal human skin sections. Most common melanocytic nevi do not express WT1, whereas Spitz nevi and dysplastic nevi show cytoplasmic WT1 staining. (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Epigenetics & Nuclear Function
Transcription Factors
Target description
Physical form
Storage and Stability
Other Notes
Legal Information
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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