The recommended guanidine concentration for Amyloid Beta Immunoassays should be less than 0.5M in the final sample concentration.
HNABTMAG-68K
MILLIPLEX® Human Amyloid Beta and Tau Magnetic Bead Panel - Multiplex Assay
allows quantitative multiplex detection of multiple analytes simultaneously
Synonym(s):
Human Neuroscience Protein Multiplex Assay, Luminex® Human Neuroscience Panel, Millipore Human Neuroscience Immunoassay
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
Recommended Products
Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 109%
(Spike recovery for Aβ1-42)
accuracy: 91%
(Spike recovery for pTau181)
accuracy: 98%
(Spike recovery for tTau)
sensitivity: 1.5 pg/mL
(pTau181; MinDC+2SD)
sensitivity: 10.2 pg/mL
(A?1-40; MinDC+2SD)
sensitivity: 14.2 pg/mL
(tTau; MinDC+2SD)
sensitivity: 2.5 pg/mL
(A?1-42; MinDC+2SD)
standard curve range: 0.7-500 pg/mL
(pTau181)
standard curve range: 11-8000 pg/mL
(tTau)
standard curve range: 2-2000 pg/mL
(Aβ1-42)
standard curve range: 21-15000 pg/mL
(Aβ1-40)
inter-assay cv: <15%
intra-assay cv: <10%
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
MILLIPLEX® Human Amyloid Beta and Tau Panel enables you to focus on the relevance and therapeutic potential of Aβ40, Aβ42, Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181) in your research model. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
The MILLIPLEX® Human Amyloid Beta Tau Bead Panel is to be used for the simultaneous quantification of Aβ40, Aβ42, tTau, and pTau181. This kit may be used only for the analysis of cerebrospinal fluid (CSF). This is a configurable assay, and some or all analytes can be selected: tau (total), amyloid β40, amyloid β42 and tau (pThr181).
MILLIPLEX® portfolio offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.
Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel
MILLIPLEX® Human Amyloid Beta Tau Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate, and build the most comprehensive library available for protein detection and quantitation.
Panel Type: Neuroscience
The MILLIPLEX® Human Amyloid Beta Tau Bead Panel is to be used for the simultaneous quantification of Aβ40, Aβ42, tTau, and pTau181. This kit may be used only for the analysis of cerebrospinal fluid (CSF). This is a configurable assay, and some or all analytes can be selected: tau (total), amyloid β40, amyloid β42 and tau (pThr181).
MILLIPLEX® portfolio offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.
Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel
MILLIPLEX® Human Amyloid Beta Tau Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate, and build the most comprehensive library available for protein detection and quantitation.
Panel Type: Neuroscience
Specificity
Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Application
- Analytes: Tau (Total), Amyloid β40, Amyloid β42, Tau (pThr181)
- Recommended Sample type: cerebrospinal fluid (CSF)
- Recommended Sample dilution: 1:2 sample dilution is recommended for extracted CSF samples
- Assay Run Time: Overnight
- Research Category: Neuroscience Metabolism
- Research Subcategory: Metabolic Disorders Obesity Inflammation & Autoimmune Mechanisms
- Research Category Neuroscience Metabolism
- NOTE: This is a competitive assay
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
96-well plate
Everything you need in a single kit.
Components
Human ABeta and Tau Standard, 1 vial, lyophilized
Human ABeta and Tau Quality Control 1, 1 vial, lyophilized
Human ABeta and Tau Quality Control 2, 1 vial, lyophilized
Bead Diluent, 1 bottle, 3.5mL
Set of one 96-Well Plate with 2 sealers
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Human ABeta and Tau Detection Antibodies, 1 bottle, 3.2 mL
Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
Mixing Bottle, 1 bottle
Human ABeta and Tau Quality Control 1, 1 vial, lyophilized
Human ABeta and Tau Quality Control 2, 1 vial, lyophilized
Bead Diluent, 1 bottle, 3.5mL
Set of one 96-Well Plate with 2 sealers
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Human ABeta and Tau Detection Antibodies, 1 bottle, 3.2 mL
Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL
Mixing Bottle, 1 bottle
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Emma L Robertson et al.
Alzheimer's & dementia (New York, N. Y.), 8(1), e12289-e12289 (2022-04-14)
Monkeys are becoming important translational models of neurodegenerative disease. To facilitate model development, we measured cerebrospinal fluid (CSF) concentrations of key biomarkers in healthy male and female cynomolgus and rhesus macaques. Amyloid beta (Aβ40, Aβ42), tau (total tau [t-tau], phosphorylated
Susan E Boehnke et al.
Alzheimer's & dementia (Amsterdam, Netherlands), 12(1), e12069-e12069 (2020-07-23)
Neurofilament light (NFL) in cerebrospinal fluid (CSF) is elevated in neurodegenerative disease patients, and may track disease progression and treatment. Macaque monkeys are emerging as important translational models of neurodegeneration, and NFL may be a useful biomarker. To determine the
Mohamed Raâfet Ben Khedher et al.
Alzheimer's & dementia (New York, N. Y.), 7(1), e12124-e12124 (2021-02-05)
In brain, extracellular vesicles (EVs) play an essential role in the neuron-glia interface and ensure the crosstalk between the brain and the periphery. Some studies now link the pathway dysfunction of the EVs to apolipoprotein E gene variant (APOE ε4)
Jessica L Hoffman et al.
International review of neurobiology, 148, 169-230 (2019-11-18)
Alzheimer's disease (AD) is a progressive neurodegenerative disorder that represents the most common cause of dementia in the United States. Although the link between alcohol use and AD has been studied, preclinical research has potential to elucidate neurobiological mechanisms that
Wencheng Yin et al.
Frontiers in neuroscience, 14, 602642-602642 (2021-01-05)
Alzheimer's disease (AD)-related degenerative decline is associated to the presence of amyloid beta (Aβ) plaque lesions and neuro fibrillary tangles (NFT). However, the precise molecular mechanisms linking Aβ deposition and neurological decline are still unclear. Here we combine genome-wide transcriptional
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What is the recommended concentration of guanidine for Amyloid Beta Immunoassays?
1 answer-
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Are the antibodies used in the Human Amyloid Beta/Tau (HNABTMAG-68K) kit also able to recognize rat samples?
1 answer-
The AB40 and AB42 antibodies have not been specifically tested for reactivity to rat. However, in a test involving 4 rat serum samples, AB40 was detected in all of the samples, while AB42 was not detectable. Additionally, the capture antibodies for pTau and Total Tau have been found to be reactive to both human and rat, as pTau and Total Tau were detected in 2 out of the 4 rat serum samples tested. It's important to note that the panel has not been tested with rat standards to determine the extent of cross-reactivity.
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Is it possible to add other analytes to the Human Amyloid Beta / Tau Panel (HNABTMAG-68K) as SPR kits?
1 answer-
Additional analytes cannot be added to the AB/Tau panel due to differences in the assay procedure. The detection antibody is added at the primary incubation, whereas the other panels have the detection added after the primary incubation.
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Can the Human Amyoid Beta / Tau (HNABTMAG-68K) Milliplex kit, which currently only lists CSF as a sample type, be used with serum/plasma samples?
1 answer-
The HNABTMAG-68K Milliplex kit currently only lists cerebrospinal fluid (CSF) as a sample type. When serum/plasma samples were tested with this kit, a strong matrix effect was observed. As the kit was not validated for serum/plasma, the appropriate Serum Matrix for use could not be ascertained. Furthermore, the expected trend for Amyloid β40 and Amyloid β42 in serum/plasma was not observed.
For serum/plasma samples, we recommend using the validated ELISA kits for Amyloid β40 and 42 (EZHS and EZH42) as they are the preferred assays. Additionally, we offer a Total Tau ELISA (RAB1085).
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Could you provide the plate capacity for an LX200 or FM3D instrument when using one box of Sheath Fluid?
1 answer-
The FLEXMAP 3D and LX200 instruments use approximately 10 mL of sheath per sample run when running a 96-well plate and the xPONENT protocol is set to aspirate 50 µL per sample. Therefore, to acquire one 96-well plate, the instruments will use approximately 1L of sheath fluid.
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Can you provide information on the specificity or cross reactivity of Total Tau in the human HNABTMAG-68K?
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The human panel HNABTMAG-68k follows the Tau standard with isoform Tau441. Both the capture and detection immunogens used are also Tau441. Cross reactivity for other isoforms has not been tested.
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How should the plate for the complete Milliplex assay in Sheath Fluid be stored if it can't be read for a couple of hours?
1 answer-
The plate can be stored at 4°C without shaking for up to 24 hours. Before reading, the plate should be shaken at room temperature for at least 20 minutes to bring it back to ambient temperature.
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