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EPA100

Millipore

EPA Peroxidase Protection Buffer

500 mL EPA Peroxidase Protection Buffer for ELISA.

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About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

form

liquid

packaging

pkg of 500 mL

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable

shipped in

wet ice

General description

Peroxidase Protective Buffer is a diluent formulated to stabilize the activity of Chemicon horseradish peroxidase (HRP)-antibody conjugates at high conjugate dilution for extended periods.

Application

USE:

Chemicon HRP conjugated antibodies may be diluted up to 1:16,000 in Peroxidase Protective Buffer. Diluted conjugates are stable for at least 3 months when stored at 2 - 8oC. The buffer is suitable for direct use in ELISA. It is recommended that the HRP conjugated antibody is diluted with Peroxidase Protective Buffer and allowed to stand for 24 hours at 2-8°C to attain constant stability before use.

Peroxidase Protective Buffer is compatible with blocking agents such as skim milk powder, Tween 20 and normal serum. However HRP conjugates should not be stored in the presence of these blocking agents. They should be added just prior to use in the assay.

Physical form

Peroxidase Protective Buffer contains 0.5% (w/v) bovine serum albumin, antimicrobial preservatives and stabilizing compounds in phosphate buffered saline, pH 7.2.

Storage and Stability

Store at +2 to +8°C, for up to 6 months.

PRECAUTIONS

HRP conjugates should be diluted and stored under clean conditions to reduce the level of microbial challenge.

Sodium azide must NOT be added to Peroxidase Protective Buffer.

Avoid contamination of HRP conjugate solutions with heavy metal ions, aromatic chlorocarbons or hypochlorous acids. Avoid containers made of soda glass or polystyrene. Storage of diluted conjugates in borosilicate glass is recommended.

Efficient washing steps should follow HRP conjugate incubations. In ELISA, at least four thorough wash cycles are recommended.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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