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Key Documents

ABE1408

Sigma-Aldrich

Anti-DNA polymerase beta Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

DNA polymerase beta, DNA polymerase beta

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

species reactivity (predicted by homology)

canine (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), feline (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

bovine ... Polb(614688)
cat ... Polb(101099797)
dog ... Polb(494001)
frog ... Polb(448741)
human ... POLB(5423)
mouse ... Polb(18970)
rat ... Polb(29240)
rhesus monkey ... Polb(704801)

General description

DNA polymerase beta (EC 2.7.7.7; UniProt P06746; also known as DNA pol beta, DNA polymerase beta subunit) is encoded by the POLB gene (Gene ID 5423) in human. DNA polymerase beta is involved in DNA recombination and base-excision repair (BER). Upon oxidative DNA damage, Polb translocates to the nucleus, where it associates with AP endonuclease 1 (Apex) to carry out DNA repair. Apex makes a single nick 5′ to an abasic site in double-stranded DNA, Polb then inserts the replacement nucleotide(s). Aberrant POLB overexpression results in an increased rate of spontaneous mutagenesis and a highly mutagenic tolerance phenotype. Thirty percent of all tumors are reported to harbor mutations in the POLB gene.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human DNA polymerase beta.

Application

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected DNA polymerase beta in normal rat testis, normal human kidney, as well as human lung & prostate cancer tissue sections.
This Anti-DNA polymerase beta Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of DNA polymerase beta.

Quality

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DNA polymerase beta in 10 µg of human and mouse testis tissue, Jurkat, and HeLa lysates.

Target description

~38 kDa observed. Uncharacterized band(s) may appear in some lysates.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jin Zheng et al.
Acta neuropathologica communications, 8(1), 25-25 (2020-03-07)
Insoluble intracellular aggregation of tau proteins into filaments and neurodegeneration are histopathological hallmarks of Alzheimer disease (AD) and other tauopathies. Recently, prefibrillar, soluble, oligomeric tau intermediates have emerged as relevant pathological tau species; however, the molecular mechanisms of neuronal responses
Neuronal enhancers are hotspots for DNA single-strand break repair.
Wu, et al.
Nature, 593, 440-444 (2023)

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