AB1554
Anti-Nerve Growth Factor Receptor Antibody, p75
serum, Chemicon®
Synonym(s):
CD271 antigen, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, low affinity nerve growth factor receptor, nerve growth factor receptor, nerve growth factor receptor (TNFR superfamily, member 16), p75 IC
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All Photos(5)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NGFR(4804)
General description
Nerve Growth Factor Receptor p75 (UniProt: Q9Z0W1; also known as Tumor necrosis factor receptor superfamily member 16, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, CD271) is encoded by the Ngfr (also known as Tnfrsf16) gene in murine species. Nerve Growth Factor Receptor p75 is a single-pass type I, homodimeric membrane protein that can serve as a low affinity receptor for NGF, BDNF, NT-3, and NT-4. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 22-246), a transmembrane domain (aa 247-265), and a cytoplasmic domain (aa 266-417). Its expression is shown to oscillate in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain and in liver and expression is seen at higher levels during the light period and lower during dark period. Depending on the developmental stage, cell type, and whether they are unbound or ligand bound, and the type of ligand, p75 can activate apoptosis or survival. During development, it signals toward apoptosis to eliminate unnecessary neurons. Its death domain is localized to amino acids 346-411, which is responsible for its interaction with RAN-binding protein 9 (RANBP9). Neurotrophin binding to p75 can activate the MAP kinase pathway leading to cell survival or apoptosis depending on the cell type or ligand type.
Specificity
It has been reported that this antibody does not recognize rat p75 by Western blot. See MAB365 for rat reactive antibody using Western blotting.
p75 nerve growth factor receptor (p75 Neurotrophin receptor, low affinity neurotrophin receptor), extracellular domain.
Immunogen
Epitope: extracellular
Recombinant mouse NGF Receptor p75 extracellular fragment.
Application
Anti-Nerve Growth Factor Receptor Antibody, p75 is an antibody against Nerve Growth Factor Receptor for use in IC, IH(P),WB and IP.
Immunoprecipitation:
1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.
Immunohistochemistry(paraffin):
1:50 and 1:300 dilution from a previous lot detected NGF Receptor p75 using IHC-Select Detection with HRP-DAB.
Immunocytochemistry:
1:500 dilution from a previous lot detected NGF Receptor p75 in rat PC12 cells.
1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.
Immunohistochemistry(paraffin):
1:50 and 1:300 dilution from a previous lot detected NGF Receptor p75 using IHC-Select Detection with HRP-DAB.
Immunocytochemistry:
1:500 dilution from a previous lot detected NGF Receptor p75 in rat PC12 cells.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neurochemistry & Neurotrophins
Quality
Routinely evaluated by immunoprecipitation on PC12 cell lysates.
Immunoprecipitation (IP): 1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.
Immunoprecipitation (IP): 1:500 - 1:5000 dilution of this antibody immunoprecipitated NGF Receptor p75 from 10 µg of PC12 cell lysate.
Target description
44.7 kDa
Linkage
Replaces: 04-1111
Physical form
Rabbit polyclonal serum containing 0.05% NaN3.
Unpurified
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Rat PC12 cells.
Rat PC12 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Alexander Schulz et al.
Acta neuropathologica, 132(2), 289-307 (2016-05-30)
Schwannomas are predominantly benign nerve sheath neoplasms caused by Nf2 gene inactivation. Presently, treatment options are mainly limited to surgical tumor resection due to the lack of effective pharmacological drugs. Although the mechanistic understanding of Nf2 gene function has advanced
Norianne T Ingram et al.
PloS one, 11(4), e0153394-e0153394 (2016-04-15)
The unique glia located in the olfactory system, called olfactory ensheathing cells (OECs), are implicated as an attractive choice for transplantation therapy following spinal cord injury because of their pro-regenerative characteristics. Adult OECs are thought to improve functional recovery and
Victor Vendrell et al.
Development (Cambridge, England), 142(16), 2792-2800 (2015-07-15)
Transcriptional regulatory networks are essential during the formation and differentiation of organs. The transcription factor N-myc is required for proper morphogenesis of the cochlea and to control correct patterning of the organ of Corti. We show here that the Otx2
NMDA-dependent switch of proBDNF actions on developing GABAergic synapses.
Langlois, A; Diabira, D; Ferrand, N; Porcher, C; Gaiarsa, JL
Cerebral Cortex (1991)
Beta-amyloid(1-42) induces neuronal death through the p75 neurotrophin receptor.
Sotthibundhu, A; Sykes, AM; Fox, B; Underwood, CK; Thangnipon, W; Coulson, EJ
The Journal of Neuroscience null
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