05-1319
Anti-EZH2 Antibody, clone BD43
clone BD43, from mouse
Synonym(s):
Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, enhancer of zeste 2, enhancer of zeste homolog 2, ENX-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BD43, monoclonal
species reactivity
human, mouse
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... EZH2(2146)
General description
EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. EZH2 is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are coexpressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene
Specificity
Not tested in other species.
This antibody recognizes human EZH2 (~98kDa).
Immunogen
Recombinant fusion protein of amino acids 1-286 of human EZH2 tagged with MBP.
Application
Research Category
Epigenetics & Nuclear Function
Apoptosis & Cancer
Epigenetics & Nuclear Function
Apoptosis & Cancer
Research Sub Category
Chromatin Biology
Chromatin Biology
Use Anti-EZH2 Antibody, clone BD43 (Mouse Monoclonal Antibody) validated in WB to detect EZH2 also known as Lysine N-methyltransferase 6, enhancer of zeste homolog 2, ENX-1.
Quality
Western Blot Analysis: 1:5,000 from a previous lot detected EZH2 of HeLa Nuclear extract.
Target description
~98 kDa
Linkage
Replaces: 04-1047
Physical form
Format: Purified
Protein G Purified
Purified immunoglobulin in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HeLa Nuclear Extracts
HeLa Nuclear Extracts
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Baocheng Zhu et al.
Cancer cell international, 21(1), 70-70 (2021-01-24)
Gastric cancer is one of the most prevalent and deadly malignancies without efficient treatment option. This study aimed to investigate the effect of hydrogen gas on the behavior of gastric cancer cells. Gastric cancer cell lines MGC-803 and BGC-823 were
Adrian P Bracken et al.
Genes & development, 20(9), 1123-1136 (2006-04-19)
The Polycomb group (PcG) proteins form chromatin-modifying complexes that are essential for embryonic development and stem cell renewal and are commonly deregulated in cancer. Here, we identify their target genes using genome-wide location analysis in human embryonic fibroblasts. We find
Su H Park et al.
Science advances, 7(15) (2021-04-09)
Forkhead box protein A1 (FOXA1) is essential for androgen-dependent prostate cancer (PCa) growth. However, how FOXA1 levels are regulated remains elusive and its therapeutic targeting proven challenging. Here, we report FOXA1 as a nonhistone substrate of enhancer of zeste homolog
Takeshi Ueda et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(37), 10370-10375 (2016-09-01)
Polycomb repressive complex 2 (PRC2) catalyzes the monomethylation, dimethylation, and trimethylation of histone H3 Lys27 (H3K27) and acts as a central epigenetic regulator that marks the repressive chromatin domain. Embryonic ectoderm development (EED), an essential component of PRC2, interacts with
Ah-Young So et al.
PloS one, 6(5), e19503-e19503 (2011-05-17)
Epigenetic regulation of gene expression is well known mechanism that regulates cellular senescence of cancer cells. Here we show that inhibition of DNA methyltransferases (DNMTs) with 5-azacytidine (5-AzaC) or with specific small interfering RNA (siRNA) against DNMT1 and 3b induced
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