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G003765

HPLC Analysis of Hydrocortisone in Topical Cream on Discovery® HS C18 after SPE using Discovery® DSC-Si

application for SPE, application for HPLC

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About This Item

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample addition: hydrocortisone topical hand cream in hexane:ethylacetate
SPE tube/cartridge: Discovery DSC-Si, 500 mg/3 mL (52695-U)
condition: 1 mL hexane:ethyl acetate (2:1, v/v)
sample addition: 1 mL
washing: 1 mL hexane:ethyl acetate (2:1, v/v)
elution: 1 mL methanol:DI water (1:1, v/v)
eluate post-treatment: dilute eluate to 2 mL with methanol:DI water (1:1, v/v)
column: Discovery HS C18, 15 cm x 4.6 mm I.D., 5 μm particles (568520-U)
mobile phase: [A] methanol; [B] DI water (1:1, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: UV, 220 nm
injection: 10 μL

suitability

application for HPLC
application for SPE

application(s)

cleaning products
cosmetics
personal care
pharmaceutical (small molecule)

Application

Sample Pre-treatment:
Dissove 1 g 1% hydrocortisone topical hand cream in 10 mL ethyl acetate. Dilute 2 mL of ethyl acetate-cream sample with 8 mL hexane:ethyl acetate (2:1, v/v).

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

SPE tube or plate

analytical column


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