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SAB4200002

Sigma-Aldrich

Anti-POU1F1/PIT1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-GHF-1, Anti-Pituitary-specific positive transcription factor 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~32 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5 μg using GH3 cell lysates
western blot: 1-2 μg/mL using GH3 cell lysates

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... POU1F1(5449)

Related Categories

General description

POU1F1 is encoded by the gene mapped to human chromosome 3p11.2.
Pituitary-specific positive transcription factor 1 (POU1F1/PIT1) is a pituitary-specific transcription factor and is a member of the POU family of transcription factors.

Specificity

Anti-POU1F1/PIT1 recognizes human POU1F1/PIT1.

Application

Anti-POU1F1/PIT1 antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Pituitary-specific positive transcription factor 1 (POU1F1/PIT1) is responsible for pituitary development and hormone expression in mammals. It regulates mammalian development. POU1F1/PIT1 is required for the differentiation and proliferation of the anterior pituitary somatotrophs, lactotrophs and thyrotrophs cell lineages. Mutation in Pit-1 gene is associated with the syndrome of combined pituitary hormone deficiency (CPHD), a disease characterized by the lack of prolactin, growth hormone and thyroid stimulating hormone β. POU1F1/PIT1 regulates its target gene expression by binding to response elements on their promoter regions and recruitment of co-regulatory proteins, that alter histone acetylation and modify chromatin structures.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shusuke Akamatsu et al.
Nature genetics, 44(4), 426-429 (2012-03-01)
We have previously reported multiple loci associated with prostate cancer susceptibility in a Japanese population using a genome-wide association study (GWAS). To identify additional prostate cancer susceptibility loci, we genotyped nine SNPs that were nominally associated with prostate cancer (P
Growth rate and carcass quality in pigs as related to genotype at loci POU1F1/RsaI (Pit1/RsaI) and GHRH/AluI
Pierzchala M, et al.
Animal Science Journal = Nihon Chikusan Gakkaiho, 21(3), 159-166 (2003)
The Pit-1beta domain dictates active repression and alteration of histone acetylation of the proximal prolactin promoter
Diamond SE and Gutierrez HA
The Journal of Biological Chemistry, 275(40), 30977-30986 (2000)
Pituitary transcription factors in the aetiology of combined pituitary hormone deficiency
Pfaffle R and Klammt J
Best Practice & Research. Clinical Endocrinology & Metabolism, 25(1), 43-60 (2011)
Evolutionary and functional analysis of the key pluripotency factor Oct4 and its family proteins
Zhang X, et al.
Journal of Genetics and Genomics = Yi Chuan Xue Bao, 40(8), 399-412 (2013)

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