The capture and detection antibodies are monoclonal Mouse IgG. The hemoglobin antibody was raised in mouse using human hemoglobin as the immunogen. The kit will only be able to detect hemoglobin in serum/plasma, so it cannot be used to detect total hemoglobin in whole blood.
Pricing and availability is not currently available.
Recommended Products
species reactivity
human
technique(s)
ELISA: suitable
assay range
inter-assay cv: <10%
intra-assay cv: <12%
shipped in
wet ice
storage temp.
−20°C
General description
The antibody pair provided in this kit recognizes Human Hemoglobin. Hemoglobin (Hb) is an iron-containing metalloprotein, which is highly expressed in red blood cells. In addition, it is also expressed in nonerythroid cells such as epithelial cells of different origin.[1] Hb is a tetramer composed of two α-globin chains and two β-globin chains. α-globin and β-globin chains is encoded by a gene mapped to human chromosome 16 and 11, respectively.[1]
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Hemoglobin (Hb) serves as the primary means of oxygen transport in vertebrates.[1] Hb exhibits antioxidant and antimicrobial activity. Alterations in blood hemoglobin levels occur in many diseases such as anemia and polycythemia.[2][3] In addition, abnormalities in Hb concentration might be implicated in pathologies associated with various respiratory diseases, such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive lung disease (COPD).[1]
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Could you please confirm if the RAB0675 kit is capable of detecting total hemoglobin? Additionally, I would like to know the types of capture and detection antibodies used in this kit.
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