MBD0001

Microbial DNA standard from Akkermansia muciniphila

Suitable for PCR, sequencing and NGS, 10 ng/μL

• UNSPSC Code: 41105500
• NACRES: NA.24

Properties

• Quality Level: 200
• form: liquid
• concentration: 10 ng/μL
• technique(s): DNA extraction: suitable; DNA sequencing: suitable; PCR: suitable
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.¹ Akkermansia muciniphila genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.
Akkermansia muciniphila is a gram negative, anaerobic, non motile, non spore forming and oval shaped bacterium. A. muciniphila inhabits the gastrointestinal tracts of more than 90% of adults and constitutes 1 to 4% of the fecal microbiota.² A. muciniphila level was suggested to be a biomarker for a healthy intestin. ³
A. muciniphila degrades mucus and utilizes it as a carbon/nitrogen source. Consequently, the host produces additional mucus while the bacterium produces oligosaccharides and Short Chain Fatty Acids (SCFAs) that can be utilized by the host and trigger the immune system. An additional protective effect of the SCFA is stimulation of mucus-associated microbiota growth, that serves as a barrier against penetration of pathogens to intestinal cells. ^2,4

Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Suitable for Quantitative standard for PCR, Sequencing and NGS

Features and Benefits

• Individual microbial standard for microbiomics and meta-genomics workflow
• Suitable standard for PCR, sequencing and NGS
• Improve Bioinformatics analyses
• Increases reproducibility
• Compare results lab to lab

Physical form

Liquid - The genomic DNA is provided at ≥10 ng/μL concentration in TE buffer pH 8.0

Other Notes

It is recommended to avoid freeze thaw cycles of this product.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable