MAK498
Alcohol Dehydrogenase (ADH) Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.05
Pricing and availability is not currently available.
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Quality Level
detection method
colorimetric
relevant disease(s)
gastrointestinal diseases
shipped in
wet ice
storage temp.
−20°C
General description
Alcohol dehydrogenase (ADH) is an oxidoreductase which catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the break down of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation.
Features and Benefits
- Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for alcohol dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)
Principle
The non-radioactive, colorimetric Alcohol Dehydrogenase (ADH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
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