This kit has not been tested on cell or tissue lysates, and its effectiveness for this sample type is unknown. It is essential to conduct tests on this specific sample. Lysates can be prepared by dissolving directly in the assay buffer, which contains detergent. The required amounts of tissue, cells, and assay buffer should be determined by the end user. As a starting guideline, use 1 million cells (or a small amount of tissue) in 100 µL of assay buffer. Homogenize with a Dounce homogenizer, centrifuge at 10,000 g for 5 minutes, and use the supernatant for the assay.
MAK366
Total Sulfite Assay Kit (Colorimetric)
sufficient for 100 colorimetric tests
Synonym(s):
Sulfite Colorimetric Assay Kit
About This Item
Recommended Products
usage
sufficient for 100 colorimetric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric
relevant disease(s)
pulmonary disorders
storage temp.
−20°C
General description
Suitability
Principle
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
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what is the best way to lyse cells/tissue for using this kit?
1 answer-
Helpful?
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What is the best way to lyse cells/tissue for using this kit?
1 answer-
This kit has not been tested on cell or tissue lysates, and its effectiveness for this sample type is unknown. It is essential to conduct tests on this specific sample. Lysates can be prepared by dissolving directly in the assay buffer, which contains detergent. The required amounts of tissue, cells, and assay buffer should be determined by the end user. As a starting guideline, use 1 million cells (or a small amount of tissue) in 100 µL of assay buffer. Homogenize with a Dounce homogenizer, centrifuge at 10,000 g for 5 minutes, and use the supernatant for the assay.
Helpful?
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How should sucrose samples be prepared to test sulfite content with kit MAK366?
1 answer-
For samples like sucrose pellets, it is advisable to dissolve or dilute them in the assay buffer and then utilize them in the assay. The sucrose should be soluble in the buffer. Metabolites present in food samples might notably impede the signal. Hence, it is suggested to dilute the samples with Sulphite Assay Buffer. In case interference persists in the diluted samples, it is recommended to prepare parallel sample well(s) as sample background control(s) and adjust the volume to 50 µl/well with Sulphite Assay Buffer. It is advised to conducting a pilot experiment and testing several samples to ensure that the readings fall within the standard curve range.
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