L4146
Leptin human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder
Synonym(s):
OB
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About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
sterility
sterile; 0.2 μm filtered
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤3.0 ng/mL ED50/EC50
mol wt
predicted mol wt ~16 kDa
impurities
≤1.0 EU/μg endotoxin
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
Gene Information
human ... LEP(3952)
General description
The gene LEP (leptin) is mapped to human chromosome 7q31. It is a 16kDa peptide. Leptin is released in the circulation and goes to the CNS (central nervous system). In CNS, it works by associating with leptin receptor.
Application
Leptin human has been used:
- to study the effect of leptin on ACAT1 (acyl-coenzyme A:cholesterol acyltransferase 1) expression and cholesterol efflux from primary cultured human macrophages
- to study the effect of leptin on expression of IGFBP2 (insulin growth factor binding protein 2) in skeletal muscle
- to study the effect of leptin on HLA-G (human leukocyte antigen G) expression in placental trophoblasts
- to study the effect of leptin on in vitro maturation, fertilization and development of horse oocytes after ICSI (intracytoplasmic sperm injection)
Biochem/physiol Actions
Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.
Human and mouse leptin share ~84% sequence identity.
Leptin is believed to play a role in normal pregnancy development.
Mutations in LEP (leptin) are linked with type-2 diabetes. Presence of high levels of leptin in plasma is related to adiposity.
Physical form
Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO3
Analysis Note
The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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