G2279

Monoclonal Anti-β-COP antibody produced in mouse

clone M3A5, ascites fluid

• Synonym(s): Anti-BARMACS, Anti-COPB
• MDL number: MFCD00164606
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: M3A5, monoclonal
• contains: 15 mM sodium azide
• species reactivity: monkey, human, chicken, goose, rabbit, canine, bovine, kangaroo rat, rat, hamster
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect immunofluorescence: 1:20 using cultured Chinese hamster ovary (CHO) cells
• isotype: IgG1
• UniProt accession no.: P53618
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... COPB1(1315) ; rat ... Copb1(114023)

Description

General description

Monoclonal Anti- β-COP (mouse IgG1 isotype) is derived from the M3A5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. COPs (coatomer proteins) have a molar mass of 110 kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles.

The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins.

Specificity

The antibody recognizes an epitope shared by β-COP (110 kDa) found in most tissue culture lines, and by a doublet of brain microtubule-associated protein (MAP2, 270-300 kDa). The antibody stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of Golgi complex and a population of coatomers scattered throughout the cytoplasm) in tissues from different species and cell processes, as well as cell bodies in chicken brain neuronal cells. It has been used for studies on the effects of various agents that influence energy status, disrupt the Golgi complex, or alter the activity of G-proteins or small GTP-binding proteins on the cellular localization of β-COP. The antibody may be used for the immunoaffinity purification of the protein.

Immunogen

microtubule-associated protein from goose brain.

Application

Monoclonal Anti-β-COP antibody produced in mouse has been used:

• for the localization of β-COP using immunoprecipitation
• in immunocytochemistry
• in immunoblotting
• with other antibodies to Golgi proteins to study the role and relationships of this protein in the cell

Biochem/physiol Actions

COPs (coatomer proteins) are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable