Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

CS0410

Sigma-Aldrich

Glutathione-S-Transferase (GST) Assay Kit

sufficient for 500 multiwell tests

Synonym(s):

GST Enzyme Activity Kit

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

EC Number:
202-551-4
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 500 multiwell tests

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... GSTA4(2941) , GSTM1(2944)

General description

The Glutathione S-Transferase (GST) Assay Kit is designed to measure total GSTactivity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma
and erythrocyte lysates

Application

The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.

Biochem/physiol Actions

Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.

Kit Components Only

Product No.
Description
  • Dulbecco's Phosphate Buffered Saline 100 mL
  • L-Glutathione Reduced 1 g
  • GST (Control) .1 mL
  • Sample Buffer 5 mL
  • Substrate (CDNB) 1.2 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000212520
098M4052V
11180225
117M4192V
11170732

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 2

1 of 2

GST GST primarily used in Western Blotting.

Sigma-Aldrich

12-350

GST

GST A1-1, Recombinant Human

Sigma-Aldrich

GS60

GST A1-1, Recombinant Human

T Lin et al.
Toxicology, 235(1-2), 1-10 (2007-04-10)
Glutathione (GSH) is one of the most important antioxidants in mammalian cells. It also plays an important role in chemical detoxification. Some evidence showed that polycyclic aromatic hydrocarbons, such as benzo[a]pyrene (B[a]P [50-32-8]), could increase GSH content as a defense
Daniel Scotcher et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(5), 556-568 (2017-03-09)
In vitro-in vivo extrapolation of drug metabolism data obtained in enriched preparations of subcellular fractions rely on robust estimates of physiologically relevant scaling factors for the prediction of clearance in vivo. The purpose of the current study was to measure
S Melino et al.
Amino acids, 41(1), 103-112 (2010-03-10)
Natural organosulfur compounds (OSCs) have been shown to have chemopreventive effects and to suppress the proliferation of tumor cells in vitro through the induction of apoptosis. The biochemical mechanisms underlying the antitumorigenic and anti-proliferative effects of garlic-derived OSCs are not
Didier Techer et al.
Environmental toxicology, 32(1), 227-240 (2015-12-18)
Gallic and pelargonic acids are biologically derived substances receiving a growing interest as eco-friendly biocides with potential applications in freshwater system management. However, some data gaps remain to address their chronic ecotoxicity issue, particularly for fish. This work aimed at
Maria Sehlstedt et al.
Particle and fibre toxicology, 7, 21-21 (2010-08-24)
Biomass combustion contributes to the production of ambient particulate matter (PM) in rural environments as well as urban settings, but relatively little is known about the health effects of these emissions. The aim of this study was therefore to characterize
View All Related Papers

Questions

1–3 of 3 Questions  

  1. Is there a protocol available for preparing tissue for assay with the CS0410 Glutathione-S-Transferase (GST) Assay Kit?

    1 answer

    1. A specific tissue preparation protocol is not included with this kit. Product C3228, CelLytic MT reagent for mammalian tissue, is suitable. Please see the link below to review the product datasheet and protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/238/c3228bul.pdf

      Flash freezing the sample in liquid nitrogen and cold homogenization methods are also suitable for use with this kit. There are a number of methods available in published literature. Please see the link below for articles that may be helpful:
      https://scholar.google.com/scholar?q=tissue+lysate+protocol&hl=en&as_sdt=0&as_vis=1&oi=scholart

      Helpful?

  2. Is this suitable for detecting all of GST activity or is there for specific GST family for example GSTA or GSTM activity only?

    1 answer

    1. This product has not been evaluated for specificity to each GST isozyme. However, there is no evidence to suggest that this kit does not react to all GST isozymes.

      Helpful?

  3. ¿Cuál es el mejor protocolo para su utilización?

    1 answer

    1. The product datasheet is listed in the DOCUMENTATION section under 'More Documents'. Please see the links below to access the Product Datasheet:
      https://www.sigmaaldrich.com/product/sigma/cs0410#product-documentation
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/301/cs0410bul.pdf

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service