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90358

Sigma-Aldrich

Triethylammonium acetate buffer

volatile buffer, ~1.0 M in H2O

Synonym(s):

Triethylammonium acetate buffer, Buffer solution 1 M pH 7.0 (volatile)

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About This Item

UNSPSC Code:
12161700
PubChem Substance ID:
24889353
NACRES:
NA.25
Pricing and availability is not currently available.

Quality Level

200

Assay

0.95—1.05 mol

form

liquid

shelf life

limited shelf life, expiry date on the label

storage condition

dry at room temperature

concentration

1 M
~1.0 M in H2O

technique(s)

electrophoresis: suitable

color

colorless

refractive index

n20/D 1.357

pH

7.0

 

6.1

density

1.002 g/mL at 20 °C

suitability

suitable for chromatography
suitable for protein modification
suitable for separation of small nucleic acid fragments

application(s)

detection
diagnostic assay manufacturing
life science and biopharma
sample preparation

storage temp.

2-8°C

InChI

1S/C6H15N.C2H4O2/c1-4-7(5-2)6-3;1-2(3)4/h4-6H2,1-3H3;1H3,(H,3,4)

InChI key

AVBGNFCMKJOFIN-UHFFFAOYSA-N

Related Categories

General description

Triethylammonium acetate, also known as TEAA, is a volatile buffer widely utilized as a buffer in various biochemical and biological applications, particularly in peptide and proteomics research. It is a common choice for ion-pairing reagent in the chromatographic separation of oligonucleotides and plays a key role in buffer solutions for oligonucleotide synthesis and purification.

Application

Triethylammonium acetate has been used:
  • as a buffer in proteomics studies[1]
  • as a component of the mobile phase to separate nucleotide sugars[2]
  • as a buffer for the separation of glycopeptides in proteomics research[3]

Features and Benefits

  • Suitable for Biological and Biochemical Research
  • Ready available solution reduce the need for preparation time

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM6149
BCCL4934
BCCL3064
BCCL2612
BCCL2613

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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Sialic acid biosynthesis pathway blockade disturbs neuronal network formation in human iPSC-derived excitatory neurons
Mijdam R et al.
Journal of Neurochemistry, 167, 76-89 (2023)
Single-molecule mRNA detection and counting in mammalian tissue
Lyubimova A, et al.
Nature Protocols, 8(9) (2013)
Mapping the O-glycoproteome using site-specific extraction of O-linked glycopeptides (EXoO)
Yang W et al.
Molecular Systems Biology, 14, e8486-e8486 (2018)
Weiming Yang et al.
Nature protocols, 15(8), 2589-2610 (2020-07-19)
Protein glycosylation is one of the most common protein modifications. A major type of protein glycosylation is O-GalNAcylation, in which GalNAc-type glycans are attached to protein Ser or Thr residues via an O-linked glycosidic bond. O-GalNAcylation is thought to play
Johannes Zander et al.
Infection and immunity, 76(4), 1333-1339 (2007-12-28)
Thymidine-dependent small-colony variants (SCVs) of Staphylococcus aureus are frequently associated with persistent and recurrent infections in cystic fibrosis patients. The phenotypic appearance of S. aureus SCVs or normal-colony variants (NCVs) is postulated to be affected by the intracellular amount of

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Oligonucleotide Standard 6 Mix LC-UV Analysis

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

Oligonucleotide Standard 6 Mix LC-UV Analysis

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hi, I want to purchase triethylammonium acetate solid, but it's not available. I wonder if this TEAA buffer made of pure TEAA, or is there anything else added to adjust the pH? Please let me know about other chemical content in this product. Thank you.

    1 answer

    1. This product is a 1M solution in water. Triethylammonium acetate is a mixture of triethylamine and acetic acid. The purity of the specific starting materials is not reported. However, the molarity is confirmed and reported in the lot specific Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/90358#product-documentation

      Helpful?

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